Chin Bull Bot ›› 2010, Vol. 45 ›› Issue (01): 79-87.doi: 10.3969/j.issn.1674-3466.2010.01.011

• 技术方法 • Previous Articles     Next Articles

Ultrastructural Changes of Egg, Zygote and Two-celled Proembryoin Sugar Beet (Beta vulgaris)

Wei Li1, Jiaheng Shen1*, Dedong Guo2, Yajia Shang1, Junping Lu1   

  1. 1College of Life Sciences and Technology, Harbin Normal University, Harbin 150025;
    2College of Life Sciences, Heilongjiang University, Harbin 150080, China
  • Received:2008-11-26 Revised:2009-03-20 Online:2010-01-01 Published:2010-01-01
  • Contact: Jiaheng Shen

Abstract:

We used transmission electron microscopy to study the ultrastructural changes in egg cell, zygote and two-celled proembryo of Beta vulgaris to provide information on the reproductive biology of angiosperms and related research into sugar beet. The mature egg cell was an inactive cell with only a few polysomes. At the early zygote stage, a large number of ribosomal precursors were produced by the nucleolus, and many polysomes appeared in the cytoplasm, which suggests a high level of metabolism. Zygote at the dormancy stage had a small nucleolus and marked decrease in ribosomes, as shown by a few polysomes, which suggests decreased metabolism. Zygotes in the prophase of mitosis and two-celled proembryo became active again in metabolism, for a prominent nucleolus, high density of ribosomes and increased number of polysomes in the cytoplasm. Polysomes change in number as “few-many-few-many” during the development of the egg, early zygote stage, zygote at dormancy, zygote in prophase of mitosis and two-celled proembryo in sugar beet, which indicates that such changes in metabolism as “low-high-low-high” activity may relate to the transformation from gametophyte to sporophyte generation.

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[1] . [J]. Chin Bull Bot, 1994, 11(专辑): 19 .
[2] Xiao Xiao and Cheng Zhen-qi. Chloroplast 4.5 S ribosomol DNA. II Gene and Origin[J]. Chin Bull Bot, 1985, 3(06): 7 -9 .
[3] CAO Cui-LingLI Sheng-Xiu. Effect of Nitrogen Level on the Photosynthetic Rate, NR Activity and the Contents of Nucleic Acid of Wheat Leaf in the Stage of Reproduction[J]. Chin Bull Bot, 2003, 20(03): 319 -324 .
[4] SONG Li-Ying TAN Zheng GAO Feng DENG Shu-Yan. Advances in in vitro Culture of Cucurbitaceae in China[J]. Chin Bull Bot, 2004, 21(03): 360 -366 .
[5] Shi Jian ming;Gui Yao-lin and Zhu Zhi-qing. Observation on Amitosis of Sugarbeet (Beta vulgaris) Petiole during Dedifferentiation in Vitro[J]. Chin Bull Bot, 1989, 6(03): 155 .
[6] LI Jun-De YANG Jian WANG Yu-Fei. Aquatic Plants in the Miocene Shanwang Flora[J]. Chin Bull Bot, 2000, 17(专辑): 261 .
[7] XU Jing-Xian WANG Yu-Fei YANG Jian PU Guang-Rong ZHANG Cui-Fen. Advances in the Research of Tertiary Flora and Climate in Yunnan[J]. Chin Bull Bot, 2000, 17(专辑): 84 -94 .
[8] Sun Zhen-xiao Xia Guang-min Chen Hui-min. Karyotype Analysis of Psathyrostachys juncea[J]. Chin Bull Bot, 1995, 12(01): 56 .
[9] . [J]. Chin Bull Bot, 1994, 11(专辑): 8 -9 .
[10] Yunpu Zheng;Jiancheng Zhao * ;Bingchang Zhang;Lin Li;Yuanming Zhang . Advances on Ecological Studies of Algae and Mosses in Biological Soil Crust[J]. Chin Bull Bot, 2009, 44(03): 371 -378 .