Identification of Cold Tolerance at the Plumule Stage Quantitative Trait Loci with Single Segment Substituted Lines in Rice

doi:10.3724/SP.J.1259.2015.00338

Abstract

Abstract: Cold tolerance at the plumule stage is an important trait during rice growth and can be easily affected by genetic background and environmental factors. Single segment substitution lines (SSSLs) can effectively reduce the interference of the genetic background. We developed a set of genome-wide SSSLs carrying Nipponbare introgression segments in the background of Guangluai 4. Quantitative trait loci (QTL) analysis of cold tolerance at the plumule stage involved one-way ANOVA and Dunnett’s test for comparing SSSLs with the recurrent parent Guangluai 4 (P≤0.001). We detected eight QTL for cold tolerance at the plumule stage on chromosomes 1, 6, 8, 9 and 10; all had a positive effect. In both years studied, the additive effect of these QTL ranged from 14% to 44% and 10% to 45%, with additive effect percentages 700% to 2 200% and 500% to 2 250%. Four of these QTL were primarily mapped by using the substitution mapping method, and qCTP9-2 had the largest additive effect in both years for 44% and 45%, respectively. These results provide useful information for exploring and using new grain number-related resources.

Jinyan Zhu, Mei Yang, Chaoqiu Ji, Jun Wang, Jie Yang, Fangjun Fan, Wenqi Li, Fangquan Wang, Guohua Liang, Yong Zhou, Weigong Zhong. Identification of Cold Tolerance at the Plumule Stage Quantitative Trait Loci with Single Segment Substituted Lines in Rice[J]. Chinese Bulletin of Botany, 2015, 50(3): 338-345.

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URL: https://www.chinbullbotany.com/EN/10.3724/SP.J.1259.2015.00338

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SSSLs	Chromosome	Substituted segment	Length (Mb)	QTL	2012		2013
SSSLs	Chromosome	Substituted segment	Length (Mb)	QTL	Additive effect (%)	Additive effect contribution (%)	Additive effect (%)	Additive effect contribution (%)
C32001	1	RM3148-RM6324	2.8	qCTP1-1	37	1 850	29	1 450
C32112	6	RM162-S6-16	5.05	qCTP6-1	41	2 050	10	500
C32129	8	RM22722	1.39	qCTP8-1	43	2 150	39	1 950
C32130	8	RM22822-RM22905	4.32	qCTP8-2	23	1 150	10	500
C32133	8	RM23175-RM23642	7.65	qCTP8-3	41	2 050	10	500
C32134	9	RM23679-RM23747	3.08	qCTP9-1	26	1 350	15	750
C32136	9	RM23679-RM23780	5.30	qCTP9-1	32	1 650	23	1 150
C32142	9	H16-RM24805	7.51	qCTP9-2	44	2 200	45	2 250
C32145	10	RM25213-S10-24	4.39	qCTP10-1	14	700	29	1 450

SSSLs	Chromosome	Substituted segment	Length (Mb)	QTL	2012		2013
SSSLs	Chromosome	Substituted segment	Length (Mb)	QTL	Additive effect (%)	Additive effect contribution (%)	Additive effect (%)	Additive effect contribution (%)
C32001	1	RM3148-RM6324	2.8	qCTP1-1	37	1 850	29	1 450
C32112	6	RM162-S6-16	5.05	qCTP6-1	41	2 050	10	500
C32129	8	RM22722	1.39	qCTP8-1	43	2 150	39	1 950
C32130	8	RM22822-RM22905	4.32	qCTP8-2	23	1 150	10	500
C32133	8	RM23175-RM23642	7.65	qCTP8-3	41	2 050	10	500
C32134	9	RM23679-RM23747	3.08	qCTP9-1	26	1 350	15	750
C32136	9	RM23679-RM23780	5.30	qCTP9-1	32	1 650	23	1 150
C32142	9	H16-RM24805	7.51	qCTP9-2	44	2 200	45	2 250
C32145	10	RM25213-S10-24	4.39	qCTP10-1	14	700	29	1 450