Chin Bull Bot ›› 2015, Vol. 50 ›› Issue (1): 22-31.doi: 10.3724/SP.J.1259.2015.00022

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Extracellular Adenosine Triphosphate Functions Downstream of Hydrogen Sulfide in Ethylene-induced Stomatal Closure in Arabidopsis thaliana

Yongmei Che, Dandan Zhang, Lixia Hou, Lanxiang Wang, Xin Liu*   

  1. Shandong Key Laboratory of Plant Biotechnology in University, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2013-11-06 Accepted:2014-04-03 Online:2015-04-09 Published:2015-01-01
  • Contact: Che Yongmei,Zhang Dandan,Liu Xin E-mail:liuxin6080@126.com
  • About author:

    ? These authors contributed equally to this paper

Abstract:

Using wild-type Arabidopsis, hydrogen sulfide (H2S)-synthesis mutants (Atl-cdes and Atd-cdes) and ABC- transporter mutants (Atmrp4, Atmrp5 and Atmrp4/5), we examined the effect of extracellular adenosine triphosphate (eATP) on ethylene-induced stomatal closure and its relationship with H2S. The ABC transporter blocker glibenclamide, P2 receptor inhibiter pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid and apyrase inhibited the inducing effects of ethylene on stomatal closure in Arabidopsis and ethylene-enhanced eATP content and upregulated relative expression of AtMRP4 and AtMRP5 in leaves of wild-type Arabidopsis but had no effect on eATP content or stomatal movement in Atmrp4, Atmrp5 and Atmrp4/5 mutants. Thus, eATP is involved in ethylene-induced stomatal closure, and AtMRP4 and AtMRP5 proteins take part in the secretion of intracellular ATP. The H2S scavenger hypotaurine inhibited the improving effect of ethylene on eATP content in leaves of wild-type Arabidopsis but had no effect on eATP content or relative expression of AtMRP4 and AtMRP5 in Atl-cdes and Atd-cdes mutants. eATP may be located downstream of H2S in ethylene-induced stomatal closure in Arabidopsis thaliana.

Effects of Gli, PPADS and Apyrase on ethylene induced stomatal closure in leaves of Arabidopsis thaliana Different lowercase letters indicated significant difference (P<0.05)"

Figure 2

Effects of Gli on ethylene-induced eATP level in seedling leaves of Arabidopsis thaliana"

Figure 4

Effects of ethylene on stomatal movement in seedling leaves of Arabidopsis thaliana Atmrp4, Atmrp5 and Atmrp4/5 Different lowercase letters indicated significant difference (P<0.05)"

Figure 3

Effects of ethylene on relative expression of AtMRP4 and AtMRP5 genes in Arabidopsis thaliana seedling leaves Different lowercase letters indicated significant difference (P<0.05)"

Figure 5

Effects of ethylene on eATP level in seedling leaves of Arabidopsis thaliana Atmrp4, Atmrp5 and Atmrp4/5 Different lowercase letters indicated significant difference (P<0.05)"

Figure 6

Effects of H2S scavenger HT on eATP level in seedling leaves of Arabidopsis thaliana Different lowercase letters indicated significant difference (P<0.05)"

Figure 7

Effects of ethylene on eATP level in seedling leaves of Arabidopsis thaliana Atl-cdes and Atd-cdes Different lowercase letters indicated significant difference (P<0.05)"

Figure 8

Effects of H2S on eATP level in seedling leaves of Atmrp4, Atmrp5 and Atmrp4/5 in Arabidopsis thaliana Different lowercase letters indicated significant difference (P<0.05)"

Figure 9

Quantitative RT-PCR analysis of H2S-induced AtMRP4 (A) and AtMRP5 (B) relative expression in leaves of Arabidopsis thaliana Different lowercase letters indicated significant difference (P<0.05)"

Figure 10

Effects of ethylene on relative expression of AtMRP4 and AtMRP5 genes in Arabidopsis thaliana leaves of Atl-cdes (A, B) and Atd-cdes (C, D) Different lowercase letters indicated significant difference (P<0.05)"

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