Chin Bull Bot ›› 2014, Vol. 49 ›› Issue (1): 30-40.doi: 10.3724/SP.J.1259.2014.00030

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Changes in Endogenous Hormone Contents and Cell Wall Component, Degrading Enzyme Activity and Their Relation in Lycium barbarum

Xiaowan Yang1, Guoqi Zheng2*, Juan Yang2, Xing Xu1, Di Lu1, Le Yang1   

  1. 1College of Agriculture, Ningxia University, Yinchuan 750021, China;

    2College of Life Science, Ningxia University, Yinchuan 750021, China
  • Received:2012-12-18 Revised:2013-02-24 Online:2014-01-01 Published:2014-01-01
  • Contact: Guoqi Zheng E-mail:zhengguoqi1977@163.com

Abstract: Using high-performance liquid chromatography, we examined the relationship between the content of endogenous hormones (zeatin [ZT], gibberellic acid [GA3], indole acetic acid [IAA], abscisic acid [ABA]) during fruit growth and development in Lycium barbarum Ningqi 1 and Ningqi 5. The diameter, length, and single-fruit weight were greater in Ningqi 5 than Ningqi 1; the early stage of fruit development, especially in the slow growth period, was a critical period for Ningqi 5 and could increase fruit size and weight. In Ningqi 1, IAA content in seeds was positively correlated with diameter and single-fruit weight and length. In Ningqi 5, ZT content in pericarp and IAA content in seeds was positively correlated with diameter and single-fruit weight. ZT can promote cell division, and in fruit growth, the cell number is more important than the cell size. In the slow-growth period (8–25 d), the content of ABA in the pericarp and seed was lower in Ningqi 5 than Ningqi 1, which explains the greater diameter, length and single-fruit weight of Ningqi 5 than Ningqi 1. The changes in GA3 and cellulase (Cx) were opposite, which indicated that Cx activity was inhibited by the high content of the pericarp GA3 in prophase and metaphase in Ningqi 1, thus showing fruit swelling. The changes of IAA in pericarp and polygalacturonase (PG), pectinesterase (PE), ZT and PE, ABA and Cx, ZT in seeds and PE were opposite. As well, in prophase and metaphase of Ningqi 5, the high content of IAA, ZT and ABA in the pericarp and ZT in seeds could promote fruit expansion, which explains the larger fruit size in Ningqi 5 than Ningqi 1. In Ningqi 5, ZT content in the pericarp and IAA content in seeds could enhance the activity of Cx. In Ningqi 1, ABA content in the pericarp could enhance the activity of PE, then promote the maturation of fruit.

[1]路安民,王美林.关于中药现代化中的物种鉴定问题-基于枸杞分类和生产问题的讨论[J].西北植物学报,2003,23(7):1077-1083.
[2] 孙志刚.宁夏枸杞药理研究进展[J].西北药学杂志,2001,16(3):133-135.
[3] HUBER D J. The role of cell wall hydrolases in fruit softening [J]. Horticultural Reviews, 1983(5):169-219.
[4] 冯美,张宁,张锦涛,张宏宝,刘听利.枸杞果实发育过程中细胞壁组分及相关酶活性的变化[J]. 西北林学院学报,2012,27(1): 40-42.
[5] 闫师杰,郭李维,吴彩娥,梁丽雅,王继栋,张丽娜.高效液相色谱法同时测定鸭梨种子中3种内源激素[J]. 分析化学(FENXIHUAXUE)研究简报,2010,6(38):843-847.
[6] 朴一龙,赵兰花,薛桂新.梨果实在贮藏过程中细胞壁成分的变化[J].果树学报,2006,23(6):880-883.
[7] 韩雅珊.食品化学实验指导[M].北京:北京农业大学出版社,1992.33.
[8] 张进献,李冬杰,张广华.不同钙处理对采后草莓果实细胞壁酶活性、果胶含量的影响[J].北方园艺, 2006,02): 24-6.
[9] 张飞,岳田利,费坚,袁亚宏,高振鹏.果胶酶活力的测定方法研究[J]. 西北农业学报, 2004,13(4):134-137
[10] 高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.
[11] 张瑞萍.纤维素酶的滤纸酶活和CMC酶活的测定[J].印染助剂,2002,05):51-53.
[12] 韩碧文.植物生长与分化[M].北京:中国农业大学,2003,2-23,226-244
[13] 薛炳烨,束怀瑞.肥城桃两品系果实细胞壁成分和水解酶活性的比较[J].园艺学报,2004,31(4):499-501.
[14] 赵云峰,林河通,林娇芬,陈绍军.果实软化的细胞壁降解酶及其调控研究进展[J]. 仲恺农业技术学院学报, 2006,19(1):65-70
[15] 李春燕,张光伦,曾秀丽,罗楠,胡强.细胞壁酶活性与甜橙果实质地的相关性研究[J].四川农业大学学报,2006,24(1):73-76
[16] 曾秀丽,张光伦,李春燕,罗楠,胡强.三个脐橙品种果实主要细胞壁酶动态变化研究[J].亚热带植物科学,2006,35(2):12-16.
[17] 陈善波.早蜜梨果实发育期间内源激素与细胞壁酶活性变化的研究[D].硕士论文,四川农业大学,2008(6).
[18] 薛炳烨,毛志泉,束怀瑞.草莓果实发育成熟过程中糖苷酶和纤维素酶活性及细胞壁组成变化[J].植物生理与分子生物学学报,2006,32(3):363~368.
[19] 王忠主编.植物生理学[M].北京:中国农业出版社,2000,264~294.
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