Chin Bull Bot ›› 2011, Vol. 46 ›› Issue (4): 396-406.doi: 10.3724/SP.J.1259.2011.00396

Previous Articles     Next Articles

H2S May Function Downstream of H2O2 in Jasmonic Acid-induced Stomatal Closure in Vicia faba

Zhihui Hou, Jing Liu, Lixia Hou, Xidong Li, Xin Liu*   

  1. College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2010-10-26 Revised:2011-01-25 Online:2011-07-01 Published:2011-07-01
  • Contact: Xin Liu
  • Supported by:

    National Natural Science Foundation of China

Abstract: Pharmacological treatments combined with laser scanning confocal microscopy (LSCM) and spectrophotography were used to study the role of H2S and H2O2 in the signaling transduction during stomatal movement responding to jasmonic acid (JA) in Vicia faba. Inhibitors of H2S synthesis (aminooxy acetic acid, hydroxylamine, and potasium pyruvate + ammonia), the scavenger of H2O2 (ascorbic acid), and the inhibitors of H2O2 synthesis (salicylhydroxamic acid, diphenylene iodonium) all reduced JA-induced stomatal closure. Moreover, JA enhanced H2O2 and H2S levels and L-/D-cysteine desulfhydrase activity in leaves and guard cells. The inhibitors of L-/D-cysteine desulfhydrase diminished JA-induced H2S production in leaves. In addition, H2O2 scavenger decreased H2S level and L-/D-cysteine desulfhydrase activity induced by JA. Therefore, H2S and H2O2 are involved in the signal transduction pathway of JA-induced stomatal closure. L-/D-cysteine desulfhydrase-derived H2S may represent a novel downstream component of the H2O2 signaling cascade during JA-induced stomatal movement in V. faba.

CLC Number: 

  • Q945

安国勇, 宋纯鹏, 张骁, 荆艳彩, 阳冬梅, 黄美娟, 周培爱, 吴才宏 (2000). 过氧化氢对蚕豆气孔运动和质膜K+通道的影响. 植物生理学报 26, 458-464.
薄惠, 王棚涛, 董发才, 宋纯鹏 (2005). 拟南芥保卫细胞中茉莉酸甲酯诱导的H2O2 产生与MAPK 信号转导体系的可能关系. 植物生理学通讯 41, 439-443.
崔香环, 苗雨晨, 董发才, 宋纯鹏 (2001). 茉莉酸甲酯诱导气孔关闭的信号转导机制初探. 河南大学学报(自然科学版) 31, 75-78.
李东波, 肖朝霞, 刘灵霞, 王金成, 宋国力, 毕玉蓉(2010). 外源硫化氢对豌豆根尖及其边缘细胞的影响. 植物学报45, 354-362.
刘国华, 侯丽霞, 刘菁, 刘新, 王学臣 (2009). H2O2介导的NO合成参与乙烯诱导的拟南芥叶片气孔关闭. 自然科学进展 19, 841-851.
刘新, 李云, 孟繁霞, 张蜀秋 (2007). H+参与茉莉酸调控蚕豆气孔运动的信号转导. 植物生理学通讯 43, 245-249.
刘新, 石武良, 张蜀秋, 娄成后 (2005). 一氧化氮参与茉莉酸诱导蚕豆气孔关闭的信号转导. 科学通报 50, 453-458.
左照江, 张汝民, 高岩 (2009). 植物间挥发物信号的研究进展. 植物学报44, 245-252.
Angelini R, Tisi A, Rea G, Chen MM, Botta M, Federico R, Cona A (2008). Invovement of polyamine oxidase in wound healing. Plant Physiol 146, 162-177.
Biswa R A, Sarah M A (2008). Hormone interactions in stomatal function. Plant Mol Biol 69, 451-462.
Bloem E, Riemenschneider A, Volker J, Papenbrock J, Schmidt A, Salac I, Haneklaus S, Schnug E (2004). Sulphur supply and infection with Pyrenopeziza brassicae in?uence L-cysteine desulphydrase activity in Brassica napus L. J Exp Bot 55, 2305-2312.
Brennan TC (1977). Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiol 59, 411-416.
Gadalla MM, Snyder SHJ (2010). Hydrogen sulfide as a gasotransmitter. Neurochem 113, 14-26.
Gehring CA, Irving HR, McConchie R, Parish RW (1997). Jasmonates induce intracellular alkalinizaton and closure of Paphiopedilum guard cells. Ann Bot 80, 485-489.
Gonugunta VK, Srivastava N, Raghavendra AS (2009). Cytosolic alkalinization is a common and early messenger preceding the production of ROS and NO during stomatal closure by variable signals, including abscisic acid, methyl jasmonate and chitosan. Plant Signal Behav 4, 561-564.
Huang DQ, Wu WR, Abrams SR, Cutler AJ (2008). The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. J Exp Bot 59, 2991-3007.
Kolla VA, Vavasseur A, Raghavendra AS (2007). Hydrogen peroxide production is an early event during bicarbonate induced stomatal closure in abaxial epidermis of Arabidopsis. Planta 225, 1421-1429.
Munemasa S, Oda K, Watanabe-Sugimoto M, NakamuraY, Shimoishi Y, Murata Y (2007). The coronatine-insensitive 1 mutation reveals the hormonal signaling interaction between abscisic acid and methyl jasmonate in Arabidopsis guard cells. Speci?c impairment of ion channel activation and second messenger production. Plant physiol 143, 1398-1407.
Papenbrock J, Riemenschneider A, Kamp A, Schulz-Vogt HN, Schmidt A (2007). Characterization of cysteine-degrading and H2S-releasing enzymes of higher plants- from the field to the test tube and back. Plant Biol 9, 582-588.
Riemenschneider A, Nikiforova V, Hoefgen R, Luit J, Kok D, Papenbrock J (2005). Impact of elevated H2S on metabolite levels, activity of enzymes and expression of genes involved in cysteine metabolism. Plant Physiol Biochem 43, 473-483.
Sekiya J, Schmidt A, Wilson LG, Filner P (1982). Emission of hydrogen sulfide by leaf tissue in response to L-Cysteine. Plant Physiol 70, 430-436.
She XP, Song XG (2008). Carbon monoxide-induced stomatai closure involves generation of hydrogen peroxide in Vicia faba guard cell. J Integr Plant Biol 50, 1539-1548.
Suhita D, Raghavendra AS, Kwak JM, Vavasseur A (2004). Cytoplasmic alkalization precedes reactive oxygen species production during methyl jasmonate- and abscisic acid-induced stomatal closure. Plant Physiol 134, 1536-1545.
Underwood W, Melotto M, He SY (2007). Role of plant stomata in bacterial invasion. Cell Microbiol 9, 1621-1629.
Veal EA, Day AM, Morgan BA (2007). Hydrogen peroxide sensing and signaling. Mol Cell 26, 1-14.
Yong QC, Choo CH, Tan BH, Low CM, Bian JS (2010).Effect of hydrogen sulfide on intracellular calcium homeostasis in neuronal cells. Neurochem Int 56, 508-515.
Zhang H, Hu LY, Hu KD, He YD, Wang SH, Luo JP (2008). Hydrogen sulfide promotes wheat seed germination and alleviates oxidative damage against copper stress. J Integr Plant Biol 50, 1518-1529.
Zhang H, Ye YK, Wang SH, Luo JP, Tang J, Ma DF (2009). Hydrogen sul?de counteracts chlorophyll loss in sweet potato seedling leaves and alleviates oxidative damage against osmotic stress. Plant Growth Regul 58, 243-250.
Zhang H, Jiao H, Jiang CX, Wang SH, Wei ZJ, Luo JP, Jones RL (2010a). Hydrogen sul?de protects soybean seedlings against drought-induced oxidative stress. Acta Physiol Plant.
Zhang H, Tan ZQ, Hu LY, Wang SH, Luo JP, Jones RL (2010b). Hydrogen sulfide alleviates aluminum toxicity in germinating wheat seedlings.J Integr Plant Biol 52, 556-567.
Zhang X, Dong FC, Gao JF, Song CP (2001). Hydrogen peroxide-induced changes in intracellular pH of guard cells precede stomatal closure. Cell Res 11, 37-43.
[1] Baozhu Li, Xiaoliang Zhao, Lei Peng. Research Advances in the Development and Regulation of Plant Chloroplasts [J]. Chin Bull Bot, 2014, 49(3): 337-345.
[2] Xueqin Wei, Changfeng Zhang, Jie Pang, Dandan Kou, Run Lan. Research Progress in the Chemistry and Structure of Plant Cuticles Related to Water Permeability [J]. Chin Bull Bot, 2013, 48(5): 573-587.
[3] Longying Wen, Tuo Chen. Changes of Pigments in Sabina przewalskii and Picea crassifolia Needles Along with Different Altitudes [J]. Chin Bull Bot, 2012, 47(4): 405-412.
[4] Chenghou Wu, Dongmei Li, Huiqiao Tian. Isolation of Zygotes and Proembryos in Doritis pulcherrima [J]. Chin Bull Bot, 2012, 47(3): 286-291.
[5] Yanming Miao, Jinzhi Lü, Runcheng Bi, Guiqin Yan. Dynamic Changes of Traits of Leaves from Elaeagnus mollis [J]. Chin Bull Bot, 2012, 47(3): 257-263.
[6] Lanxiang Wang, Zhihui Hou, Lixia Hou, Fanggui Zhao, Xin Liu. H2S Induced by H2O2 Mediates Drought-induced Stomatal Closure in Arabidopsis thaliana [J]. Chin Bull Bot, 2012, 47(3): 217-225.
Full text



[1] Yan Xiao-hua Cai Zhu-ping. Effects of S-07, PP333 and Triadimefon on Peroxidaseisoentyme of Rice Seedling[J]. Chin Bull Bot, 1995, 12(专辑3): 109 -112 .
[2] . [J]. Chin Bull Bot, 1994, 11(专辑): 13 .
[3] Xiaomin Yu;Xingguo Lan;Yuhua Li. The Ub/26S Proteasome Pathway and Self-incompatible Responses in Flowering Plants[J]. Chin Bull Bot, 2006, 23(2): 197 -206 .
[4] WANG Ling-Li LIU Wen-Zhe. Contents of Camptothecin in Camptotheca acuminata from Different Provenances[J]. Chin Bull Bot, 2005, 22(05): 584 -589 .
[5] Dai Yun-ling and Xu Chun-hui. Advances in Research on Protein Components of Oxygen-evolving Complex[J]. Chin Bull Bot, 1992, 9(03): 1 -16 .
[6] . Advances in Research on Photosynthesis of Submerged Macrophytes[J]. Chin Bull Bot, 2005, 22(增刊): 128 -138 .
[7] Shaobin Zhang;Guoqin Liu. Research Advances in Plant Actin Isoforms[J]. Chin Bull Bot, 2006, 23(3): 242 -248 .
[9] MA Li-Hui, WU Pu-Te, and WANG You-Ke. Spatial pattern of root systems of dense jujube plantation with jujube age in the semiarid loess hilly region of China[J]. Chin J Plan Ecolo, 2012, 36(4): 292 -301 .
[10] PAN Yu-De, Melillo J. M., Kicklighter D. W., XIAO Xiang-Ming, McGuire A. D.. Modeling Structural and Functional Responses of Terrestria Ecosystems in China to Changes in Climate and Atmospheric CO2[J]. Chin J Plan Ecolo, 2001, 25(2): 175 -189 .