Abstract: Using two different genotypes of Pinguicula cyclosecta and Pinguicula 'Sethos' as explants, an efficient system of adventitious bud regeneration of pinguicula was established by studying the factors affecting regeneration. The results showed that the two kinds of Pinguicula had the same requirements for disinfection scheme and basic medium, but the regeneration ability was different. The best medium for leaf regeneration of Pinguicula cyclosecta was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA, with the regeneration rate of 92.22% and the regeneration coefficient was 4.84.On MS+0.6 mg·L^-1 6-BA+0.1mg/L medium, multiplication coefficient of adventitious bud reached 4.98. The best medium for leaf regeneration of Pinguicula ‘Sethos’ was MS+2.0 mg/L 6-BA+0.2 mg/L NAA, with the regeneration rate of 77.78% and the regeneration coefficient was 6.12. On the medium MS+ 0.3 mg·L^-1 6-BA+ 0.1 mg·L^-1 NAA, the multiplication coefficient could reach 4.84. After root culture (1/2MS+ 0.1 mg·L^-1 IBA), the root state of the two kinds of Pinguicula was the best. This study solved the problems of low reproduction coefficient and difficulty in factory breeding of Pinguicula and provided technical support for its large-scale production and breeding improvement.

Key words: carnivorous plant, Pinguicula, leaf regeneration, adventitious bud