Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (2): 148-158.doi: 10.11983/CBB16087

Previous Articles     Next Articles

Research Progress in Whole-genome Analysis and Cloning of Genes Underlying Important Agronomic Traits in Soybean

Zhengjun Xia*   

  1. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China
  • Received:2016-04-19 Accepted:2016-07-08 Online:2017-04-05 Published:2017-03-01
  • Contact: Xia Zhengjun
  • About author:

    # Co-first authors


Since the soybean genome was published in Nature, in 2010, many researchers from different countries, mainly China, have re-sequenced the genomes of wild soybean accessions, landraces and cultivated cultivars of soybean. The general features at the whole-genome level during evolution and domestication have been revealed by comparative genomic analysis. Moreover, several breakthroughs have been achieved by using sequence information of the soybean genome, as evidenced by successful cloning of a number of genes underlying important agronomic traits and understanding their general regulatory mechanisms. The recent progress will lay a solid foundation for studying the elaborate regulatory network of different molecular pathways and fulfill prerequisites for breeding new cultivars with a molecular (model) design.

[1] 种康, 王台, 钱前, 王小菁, 左建儒, 顾红雅, 姜里文, 陈之端, 白永飞, 杨淑华, 孔宏智, 陈凡, 萧浪涛 (2015). 2014年中国植物科学若干领域重要研究进展. 植物学报 50, 412-459.
[2] 夏正俊 (2013). 大豆光周期反应与生育期基因研究进展. 作物学报 39, 1-9.
[3] 薛勇彪, 种康, 韩斌, 桂建芳, 王台, 傅向东, 何祖华, 储成才, 田志喜, 程祝宽, 林少扬 (2015). 开启中国设计育种新篇章——“分子模块设计育种创新体系”战略性先导科技专项进展. 中科院院刊 30, 393-402.
[4] Cao D, Li Y, Lu S, Wang J, Nan H, Li X, Shi D, Fang C, Zhai H, Yuan X, Anai T, Xia Z, Liu B, Kong F (2015a).GmCOL1a and GmCOL1b function as flowering repressors in soybean under long-day conditions. Plant Cell Physiol 56, 2409-2422.
[5] Cao D, Li Y, Wang J, Nan H, Wang Y, Lu S, Jiang Q, Li X, Shi D, Fang C, Yuan X, Zhao X, Li X, Liu B, Kong F (2015b). GmmiR156b overexpression delays flowering time in soybean.Plant Mol Biol 89, 353-363.
[6] Chan C, Qi X, Li MW, Wong FL, Lam HM (2012). Recent developments of genomic research in soybean.J Genet Genomics 39, 317-324.
[7] Cook DE, Lee TG, Guo XL, Melito S, Wang K, Bayless AM, Wang JP, Hughes TJ, Willis DK, Clemente TE, Diers BW, Jiang JM, Hudson ME, Bent AF (2012). Copy number variation of multiple genes atRhg1 mediates nematode resistance in soybean. Science 338, 1206-1209.
[8] Dong Y, Yang X, Liu J, Wang BH, Liu BL, Wang YZ (2014). Pod shattering resistance associated with domestication is mediated by aNAC gene in soybean. Nat Commun 5, 3352.
[9] Du J, Tian Z, Sui Y, Zhao M, Song Q, Cannon SB, Cregan P, Ma J (2012). Pericentromeric effects shape the patterns of divergence, retention, and expression of duplicated genes in the paleopolyploid soybean.Plant Cell 24, 21-32.
[10] Du JC, Tian ZX, Bowen NJ, Schmutz J, Shoemaker RC, Ma JX (2010). Bifurcation and enhancement of autonomous-nonautonomous retrotransposon partnership thr- ough LTR swapping in soybean.Plant Cell 22, 48-61.
[11] Fan CM, Hu RB, Zhang XM, Wang X, Zhang WJ, Zhang QZ, Ma JH, Fu YF (2014). Conserved CO-FT regulons contribute to the photoperiod flowering control in soybean.BMC Plant Biol 14, 9.
[12] Fang C, Li W, Li G, Wang Z, Zhou Z, Ma Y, Shen Y, Li C, Wu Y, Zhu B, Yang W, Tian Z (2013). Cloning ofLn gene through combined approach of map-based cloning and association study in soybean. J Genet Genomics 40, 93-96.
[13] Funatsuki H, Suzuki M, Hirose A, Inaba H, Yamada T, Hajika M, Komatsu K, Katayama T, Sayama T, Ishimoto M, Fujino K (2014). Molecular basis of a shattering resistance boosting global dissemination of soybean.Proc Natl Acad Sci USA 111, 17797-17802.
[14] Garner WW, Allard HA (1920). Effect of the relative length of day and night and other factors of the environment on growth and reproduction in plants.J Agric Res 18, 553-606.
[15] Guan RX, Qu Y, Guo Y, Yu LL, Liu Y, Jiang JH, Chen JG, Ren YL, Liu GY, Tian L, Jin LG, Liu ZX, Hong HL, Chang RZ, Gilliham M, Qiu LJ (2014). Salinity tolerance in soybean is modulated by natural variation inGmSALT3. Plant J 80, 937-950.
[16] Jang SJ, Sato M, Sato K, Jitsuyama Y, Fujino K, Mori H, Takahashi R, Benitez ER, Liu B, Yamada T, Abe J (2015). A single-nucleotide polymorphism in an endo-1, 4-β-glucanase gene controls seed coat permeability in soybean.PLoS One 10, e0128527.
[17] Jeong N, Moon JK, Kim HS, Kim CG, Jeong SC (2011). Fine genetic mapping of the genomic region controlling leaflet shape and number of seeds per pod in the soybean.Theor Appl Genet 122, 865-874.
[18] Kim KD, El Baidouri M, Abernathy B, Iwata-Otsubo A, Chavarro C, Gonzales M, Libault M, Grimwood J, Jackson SA (2015). A comparative epigenomic analysis of polyploidy-derived genes in soybean and common bean.Plant Physiol 168, 1433-1447.
[19] Kim MY, Lee S, Van K, Kim TH, Jeong SC, Choi IY, Kim DS, Lee YS, Park D, Ma J, Kim WY, Kim BC, Park S, Lee KA, Kim DH, Kim KH, Shin JH, Jang YE, Do Kim K, Liu WX, Chaisan T, Kang YJ, Lee YH, Kim KH, Moon JK, Schmutz J, Jackson SA, Bhak J, Lee SH (2010). Whole-genome sequencing and intensive analysis of the undomesticated soybean (Glycine soja Sieb. and Zucc.) genome. Proc Natl Acad Sci USA 107, 22032-22037.
[20] Kong FJ, Nan HY, Cao D, Li Y, Wu FF, Wang JL, Lu SJ, Yuan XH, Cober ER, Abe J, Liu BH (2014). A new dominant geneE9 conditions early flowering and maturity in soybean. Crop Sci 54, 2529-2535.
[21] Lam HM, Xu X, Liu X, Chen WB, Yang GH, Wong FL, Li MW, He WM, Qin N, Wang B, Li J, Jian M, Wang JA, Shao GH, Wang J, Sun SSM, Zhang GY (2010). Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection.Nat Genet 42, 1053-1059.
[22] Li QG, Zhang L, Li C, Zhang YM (2013a). Comparative genomics suggests that an ancestral polyploidy event leads to enhanced root nodule symbiosis in the Papilionoideae.Mol Biol Evol 30, 2602-2611.
[23] Li YH, Zhao SC, Ma JX, Li D, Yan L, Li J, Qi XT, Guo XS, Zhang L, He WM, Chang RZ, Liang QS, Guo Y, Ye C, Wang XB, Tao Y, Guan RX, Wang JY, Liu YL, Jin LG, Zhang XQ, Liu ZX, Zhang LJ, Chen J, Wang KJ, Nielsen R, Li RQ, Chen PY, Li WB, Reif JC, Purugganan M, Wang J, Zhang MC, Wang J, Qiu LJ (2013b). Molecular footprints of domestication and improvement in soybean revealed by whole genome re-sequencing.BMC Genomics 14, 579.
[24] Li YH, Zhou G, Ma J, Jiang W, Jin LG, Zhang Z, Guo Y, Zhang J, Sui Y, Zheng L, Zhang SS, Zuo Q, Shi XH, Li YF, Zhang WK, Hu Y, Kong G, Hong HL, Tan B, Song J, Liu ZX, Wang Y, Ruan H, Yeung CK, Liu J, Wang H, Zhang LJ, Guan RX, Wang KJ, Li WB, Chen SY, Chang RZ, Jiang Z, Jackson SA, Li R, Qiu LJ (2014). De novo assembly of soybean wild relatives for pan-genome ana- lysis of diversity and agronomic traits.Nat Biotechnol 32, 1045-1052.
[25] Liew LC, Singh MB, Bhalla PL (2014). Unique and conserved features of floral evocation in legumes.J Integr Plant Biol 56, 714-728.
[26] Liu BH, Kanazawa A, Matsumura H, Takahashi R, Harada K, Abe J (2008). Genetic redundancy in soybean photoresponses associated with duplication of the phytochrome A gene.Genetics 180, 995-1007.
[27] Liu BH, Watanabe S, Uchiyama T, Kong FJ, Kanazawa A, Xia ZJ, Nagamatsu A, Arai M, Yamada T, Kitamura K, Masuta C, Harada K, Abe J (2010). The soybean stem growth habit geneDt1 is an ortholog of Arabidopsis TERMINAL FLOWER1. Plant Physiol 153, 198-210.
[28] Liu J, Li Y, Wang W, Gai J, Li Y (2016a). Genome-wide analysis of MATE transporters and expression patterns of a subgroup of MATE genes in response to aluminum toxi- city in soybean.BMC Genomics 17, 223.
[29] Liu SM, Kandoth PK, Warren SD, Yeckel G, Heinz R, Alden J, Yang CL, Jamai A, El-Mellouki T, Juvale PS, Hill J, Baum TJ, Cianzio S, Whitham SA, Korkin D, Mitchum MG, Meksem K (2012). A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens.Nature 492, 256-260.
[30] Liu T, Fang C, Ma Y, Shen Y, Li C, Li Q, Wang M, Liu S, Zhang J, Zhou Z, Yang R, Wang Z, Tian Z (2016b). Global investigation of the co-evolution of MIRNA genes and microRNA targets during soybean domestication.Plant J 85, 396-409.
[31] Liu Y, Zhang D, Ping J, Li S, Chen Z, Ma J (2016c). Innovation of a regulatory mechanism modulating semide- terminate stem growth through artificial selection in soybean.PLoS Genet 12, e1005818.
[32] Lu X, Li QT, Xiong Q, Li W, Bi YD, Lai YC, Liu XL, Man WQ, Zhang WK, Ma B, Chen SY, Zhang JS (2016). The trans- criptomic signature of developing soybean seeds reveals genetic basis of seed trait adaptation during domestication.Plant J 86, 530-544.
[33] Pan WJ, Tao JJ, Cheng T, Bian XH, Wei W, Zhang WK, Ma B, Chen SY, Zhang JS (2016). Soybean miR172a improves salt tolerance and can function as a long distance signal.Mol Plant 9, 1337-1340.
[34] Pandey MK, Roorkiwal M, Singh VK, Ramalingam A, Kudapa H, Thudi M, Chitikineni A, Rathore A, Varshney RK (2016). Emerging genomic tools for legume breeding: current status and future prospects.Front Plant Sci 7, 455.
[35] Ping J, Liu Y, Sun L, Zhao M, Li Y, She M, Sui Y, Lin F, Liu X, Tang Z, Nguyen H, Tian Z, Qiu L, Nelson RL, Clemente TE, Specht JE, Ma J (2014). Dt2 is a gain-of- function MADS-domain factor gene that specifies semideterminacy in soybean.Plant Cell 26, 2831-2842.
[36] Qi XP, Li MW, Xie M, Liu X, Ni M, Shao GH, Song C, Yim AKY, Tao Y, Wong FL, Isobe S, Wong CF, Wong KS, Xu CY, Li CQ, Wang Y, Guan R, Sun FM, Fan GY, Xiao ZX, Zhou F, Phang TH, Liu X, Tong SW, Chan TF, Yiu SM, Tabata S, Wang J, Xu X, Lam HM (2014). Identification of a novel salt tolerance gene in wild soybean by whole- genome sequencing.Nat Commun 5, 4340.
[37] Sayama T, Ono E, Takagi K, Takada Y, Horikawa M, Nakamoto Y, Hirose A, Sasama H, Ohashi M, Hasegawa H, Terakawa T, Kikuchi A, Kato S, Tatsuzaki N, Tsukamoto C, Ishimoto M (2012). TheSg-1 glycosyltransferase locus regulates structural diversity of triterpenoid saponins of soybean. Plant Cell 24, 2123-2138.
[38] Schmutz J, Cannon SB, Schlueter J, Ma JX, Mitros T, Nelson W, Hyten DL, Song QJ, Thelen JJ, Cheng JL, Xu D, Hellsten U, May GD, Yu YS, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu SQ, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du JC, Tian ZX, Zhu LC, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010). Genome sequence of the palaeopolyploid soybean.Nature 463, 178-183.
[39] Shen Y, Zhou Z, Wang Z, Li W, Fang C, Wu M, Ma Y, Liu T, Kong LA, Peng DL, Tian Z (2014). Global dissection of alternative splicing in paleopolyploid soybean.Plant Cell 26, 996-1008.
[40] Song QX, Lu X, Li QT, Chen H, Hu XY, Ma B, Zhang WK, Chen SY, Zhang JS (2013). Genome-wide analysis of DNA methylation in soybean. Mol Plant 6, 1961-1974.
[41] Stupar RM, Specht JE (2013). Insights from the soybean (Glycine max and Glycine soja) genome: past, present, and future. Adv Agron 118, 177-204.
[42] Sun LJ, Miao ZY, Cai CM, Zhang DJ, Zhao MX, Wu YY, Zhang XL, Swarm SA, Zhou LW, Zhang ZYJ, Nelson RL, Ma JX (2015). GmHs1-1, encoding a calcineurin-like protein, controls hard-seededness in soybean.Nat Genet 47, 939-943.
[43] Tian ZX, Wang XB, Lee R, Li YH, Specht JE, Nelson RL, McClean PE, Qiu LJ, Ma JX (2010). Artificial selection for determinate growth habit in soybean.Proc Natl Acad Sci USA 107, 8563-8568.
[44] Tian ZX, Zhao MX, She MY, Du JC, Cannon SB, Liu X, Xu X, Qi XP, Li MW, Lam HM, Ma JX (2012). Genome-wide characterization of nonreference transposons reveals evolu- tionary propensities of transposons in soybean.Plant Cell 24, 4422-4436.
[45] Valliyodan B, Qiu D, Patil G, Zeng P, Huang J, Dai L, Chen C, Li Y, Joshi T, Song L, Vuong TD, Musket TA, Xu D, Shannon JG, Shifeng C, Liu X, Nguyen HT (2016). Landscape of genomic diversity and trait discovery in soybean.Sci Rep 6, 23598.
[46] Wang F, Chen HW, Li QT, Wei W, Li W, Zhang WK, Ma B, Bi YD, Lai YC, Liu XL, Man WQ, Zhang JS, Chen SY (2015a). GmWRKY27 interacts with GmMYB174 to reduce expression ofGmNAC29 for stress tolerance in soybean plants. Plant J 83, 224-236.
[47] Wang J, Chu S, Zhang H, Zhu Y, Cheng H, Yu D (2016a). Development and application of a novel genome-wide SNP array reveals domestication history in soybean.Sci Rep 6, 20728.
[48] Wang Y, Gu Y, Gao H, Qiu L, Chang R, Chen S, He C (2016b). Molecular and geographic evolutionary support for the essential role ofGIGANTEAa in soybean domestication of flowering time. BMC Evol Biol 16, 79.
[49] Wang Y, Wang L, Zou Y, Chen L, Cai Z, Zhang S, Zhao F, Tian Y, Jiang Q, Ferguson BJ, Gresshoff PM, Li X (2014). Soybean miR172c targets the repressive AP2 transcription factor NNC1 to activateENOD40 expression and regulate nodule initiation. Plant Cell 26, 4782-4801.
[50] Wang Z, Tian Z (2015). Genomics progress will facilitate molecular breeding in soybean. Sci China Life Sci 58, 813-815.
[51] Wang Z, Zhou Z, Liu Y, Liu T, Li Q, Ji Y, Li C, Fang C, Wang M, Wu M, Shen Y, Tang T, Ma J, Tian Z (2015b). Functional evolution of phosphatidylethanolamine binding proteins in soybean and Arabidopsis.Plant Cell 27, 323-336.
[52] Watanabe S, Hideshima R, Xia ZJ, Tsubokura Y, Sato S, Nakamoto Y, Yamanaka N, Takahashi R, Ishimoto M, Anai T, Tabata S, Harada K (2009). Map-based cloning of the gene associated with the soybean maturity locusE3. Genetics 182, 1251-1262.
[53] Watanabe S, Xia ZJ, Hideshima R, Tsubokura Y, Sato S, Yamanaka N, Takahashi R, Anai T, Tabata S, Kitamura K, Harada K (2011). A map-based cloning strategy employing a residual heterozygous line reveals that theGIGANTEA gene is involved in soybean maturity and flowering. Genetics 188, 395-407.
[54] Wu FQ, Price BW, Haider W, Seufferheld G, Nelson R, Hanzawa Y (2014). Functional and evolutionary characterization of theCONSTANS gene family in short-day pho- toperiodic flowering in soybean. PLoS One 9, e85754.
[55] Xia ZJ, Watanabe S, Yamada T, Tsubokura Y, Nakashima H, Zhai H, Anai T, Sato S, Yamazaki T, Lu SX, Wu HY, Tabata S, Harada K (2012a). Positional cloning and characterization reveal the molecular basis for soybean maturity locusE1 that regulates photoperiodic flowering. Proc Natl Acad Sci USA 109, E2155-E2164.
[56] Xia ZJ, Zhai H, Liu BH, Kong FJ, Yuan XH, Wu HY, Cober E, Harada K (2012b). Molecular identification of genes controlling flowering time, maturity, and photoperiod response in soybean.Plant Syst Evol 298, 1217-1227.
[57] Xia ZJ, Zhai H, Lu SX, Wu HY, Zhang YP (2013). Recent achievement in gene cloning and functional genomics in soybean. Scientific World J 2013, 281367.
[58] Xu M, Yamagishi N, Zhao C, Takeshima R, Kasai M, Watanabe S, Kanazawa A, Yoshikawa N, Liu B, Yamada T, Abe J (2015). The soybean-specific maturity geneE1 family of floral repressors controls night-break responses through down-regulation of FLOWERING LOCUS T ortho- logs. Plant Physiol 168, 1735-1746.
[59] Xu ML, Xu ZH, Liu BH, Kong FJ, Tsubokura Y, Watanabe S, Xia ZJ, Harada K, Kanazawa A, Yamada T, Abe J (2013a). Genetic variation in four maturity genes affects photoperiod insensitivity and PHYA-regulated postflowering responses of soybean.BMC Plant Biol 13, 91.
[60] Xu X, Zeng L, Tao Y, Vuong T, Wan J, Boerma R, Noe J, Li Z, Finnerty S, Pathan SM, Shannon JG, Nguyen HT (2013b). Pinpointing genes underlying the quantitative trait loci for root-knot nematode resistance in palaeopolyploid soybean by whole genome resequencing.Proc Natl Acad Sci USA 110, 13469-13474.
[61] Zeng QY, Yang CY, Ma QB, Li XP, Dong WW, Nian H (2012). Identification of wild soybean miRNAs and their target genes responsive to aluminum stress.BMC Plant Biol 12, 182.
[62] Zhai H, Lü S, Wu H, Zhang Y, Zhang X, Yang J, Wang Y, Yang G, Qiu H, Cui T, Xia Z (2015). Diurnal expression pattern, allelic variation, and association analysis reveal functional features of theE1 gene in control of photoperio- dic flowering in soybean. PLoS One 10, e0135909.
[63] Zhai H, Lu SX, Liang S, Wu HY, Zhang XZ, Liu BH, Kong FJ, Yuan XH, Li J, Xia ZJ (2014a).GmFT4, a homolog of FLOWERING LOCUS T, is positively regulated by E1 and functions as a flowering repressor in soybean. PLoS One 199, e89030.
[64] Zhai H, Lu SX, Wang YQ, Chen X, Ren HX, Yang JY, Cheng W, Zong CM, Gu HP, Qiu HM, Wu HY, Zhang XZ, Cui TT, Xia ZJ (2014b). Allelic variations at four major maturityE genes and transcriptional abundance of the E1 gene are associated with flowering time and maturity of soybean cultivars. PLoS One 9, e97636.
[65] Zhang D, Song H, Cheng H, Hao D, Wang H, Kan G, Jin H, Yu D (2014). The acid phosphatase-encoding geneGm- ACP1 contributes to soybean tolerance to low-phosph- orus stress. PLoS Genet 10, e1004061.
[66] Zhang JP, Song QJ, Cregan PB, Nelson RL, Wang XZ, Wu JX, Jiang GL (2015). Genome-wide association study for flowering time, maturity dates and plant height in early maturing soybean (Glycine max) germplasm. BMC Genomics 16, 217.
[67] Zhang XZ, Zhai H, Wang YY, Tian XY, Zhang YP, Wu HY, Lü SY, Yang G, Li YQ, Wang L, Hu B, Bo QY, Xia ZJ (2016). Functional conservation and diversification of the soybean maturity geneE1 and its homologs in legumes. Sci Rep 13, 29548.
[68] Zhao C, Takeshima R, Zhu J, Xu M, Sato M, Watanabe S, Kanazawa A, Liu B, Kong F, Yamada T, Abe J (2016). A recessive allele for delayed flowering at the soybean maturity locusE9 is a leaky allele of FT2a, a FLOWERING LOCUS T ortholog. BMC Plant Biol 16, 20.
[69] Zhao X, Cao D, Huang Z, Wang J, Lu S, Xu Y, Liu B, Kong F, Yuan X (2015). Dual functions ofGmTOE4a in the regulation of photoperiod-mediated flowering and plant morphology in soybean. Plant Mol Biol 88, 343-355.
[70] Zhou L, Wang SB, Jian JB, Geng QC, Wen J, Song QJ, Wu ZZ, Li GJ, Liu YQ, Dunwell JM, Zhang J, Feng JY, Niu Y, Zhang L, Ren WL, Zhang YM (2015a). Identification of domestication-related loci associated with flowering time and seed size in soybean with the RAD-seq genotyping method.Sci Rep 5, 9350.
[71] Zhou Z, Jiang Y, Wang Z, Gou Z, Lyu J, Li W, Yu Y, Shu L, Zhao Y, Ma Y, Fang C, Shen Y, Liu T, Li C, Li Q, Wu M, Wang M, Wu Y, Dong Y, Wan W, Wang X, Ding Z, Gao Y, Xiang H, Zhu B, Lee SH, Wang W, Tian Z (2015b). Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean.Nat Biotechnol 33, 408-414.
No related articles found!
Full text



[1] Liu De-li. Heat-Shock Proteins of Plants and their Functions[J]. Chinese Bulletin of Botany, 1996, 13(01): 14 -19 .
[2] Chengqiang Ding, Dan Ma, Shaohua Wang, Yanfeng Ding. Optimization Process and Method of 2-D Electrophoresis for Rice Proteomics[J]. Chinese Bulletin of Botany, 2011, 46(1): 67 -73 .
[3] SONG Ke-Min. Phosphorus Nutrition of Plants: Phosphate Transport Systems and their Regulation[J]. Chinese Bulletin of Botany, 1999, 16(03): 251 -256 .
[4] CHEN Fa-Ju;YANG Ying-Gen;ZHAO De-Xiu;GUI Yao-Lin and GUO Zhong-Chen. Advances in Studies of Species Habitats Distribution and Chemical Composition of Snow Lotuses(Saussurea) in China[J]. Chinese Bulletin of Botany, 1999, 16(05): 561 -566 .
[5] YANG Hong-QiangJIE Yu-lingLI Jun. The Stresses Messenger from Roots and Its Production and Transport in Plant[J]. Chinese Bulletin of Botany, 2002, 19(01): 56 -62 .
[6] Hui Li, Guangcan Zhang, Huicheng Xie, Jingwei Xu, Chuanrong Li, Juwen Sun. The Effect of Phenol Concentration on Photosynthetic Physiological Parameters of Salix babylonica[J]. Chinese Bulletin of Botany, 2016, 51(1): 31 -39 .
[7] . [J]. Chinese Bulletin of Botany, 1996, 13(专辑): 97 -98 .
[9] Wang Renqing. Comparative Study on the Vegetation Between Shandong and Liaodong Peninsulas[J]. Chin J Plan Ecolo, 1984, 8(1): 41 -51 .
[10] Ge Ying, Chang Jie, Lu Dagen, Yue Chunlei, Jiang Hong. A Study on the Ecological Characters of Mosla hangchowensis[J]. Chin J Plan Ecolo, 1999, 23(1): 14 -22 .