Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (1): 43-53.doi: 10.11983/CBB16081

Special Issue: Rice Biology

• Orginal Article • Previous Articles     Next Articles

Molecular Evolution and Expression Analysis of the OsMIP1 Response to Abiotic Stress

Wang Ling1,2, Guo Changkui1,2,3, Ren Ding1,2,*(), Ma Hong1,2,*()   

  1. 1School of Life Sciences, Fudan University, Shanghai 200438, China
    2Institute of Plant Biology, Fudan University, Shanghai 200438, China
    3School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
  • Received:2016-04-16 Accepted:2016-05-26 Online:2017-01-23 Published:2017-01-01
  • Contact: Ren Ding,Ma Hong E-mail:rending2013@163.com;hongma@fudan.edu.cn
  • About author:

    # Co-first authors

Abstract:

The gene MID1 (MYB IMPORTANT FOR DROUGHT RESPONSE1) encodes a putative R-R type MYB transcription factor; is induced by abiotic stresses, especially drought in reproductive stage; and can improve pollen fertility and rice production. To understand the role of MID1 in abiotic stress responses, we used the yeast two-hybrid system to find an interacting protein, OsMIP1 (Oryza sativa MID1 interaction protein 1). The interaction was further confirmed by BiFC (bimolecular fluorescence complementation) analysis in tobacco leaf cells. OsMIP1 encodes a putative transmembrane protein with an ENTH/ANTH/VHS domain. It is expressed in the root, stem, leaf, panicle and endosperm. Under drought stress, its expression is upregulated in leaf and reproductive organs, especially in post-meiotic flowers. OsMIP1 may play a role in response to drought stress during reproductive development. OsMIP1 expression during vegetative development can be induced by other abiotic stress, including NaCl and mannitol, which suggests that OsMIP1 can respond to other abiotic stresses. There is little analysis of the evolution of genes encoding proteins with the ENTH/ ANTH/VHS domain, so we analyzed the molecular evolution of MIP1 homologs in flowering plants. The evolution analysis of the MIP1 family in angiosperms showed that MIP1 homologs can be divided into 6 types, which originated from at least 6 copies of MIP1 homologous genes in the ancestor of extent angiosperms. After gene-duplication and -loss events, MIP1 family members widely distributed in the angiosperms and might have various functions, possibly in stress responses.

Figure 1

Analysis of conserved domains of OsMIP1 protein"

Figure 2

OsMIP1 interacts with MID1 (A) OsMIP1 interacts with MID1 in yeast; (B) BiFC (bimole- cular fluorescence complementation) analysis of OsMIP1- MID1 interaction in tobacco leaf (YFP: Yellow fluorescent protein; Merge: Merged image)"

Figure 3

Subcellular localization of OsMIP1YFP: Yellow fluorescent protein; Merge: Merged image"

Figure 4

Expression patterns of OsMIP1 in different tissuesR: Root; S: Stem; L: Leaf; MF: Meiotic floret; PMF: Post- meiotic floret; FS: 5-day-filling seeds"

Figure 5

Expression patterns of OsMIP1 in different abiotic stress(A) Expression patterns of OsMIP1 in different tissues under drought; (B) Expression patterns of OsMIP1 in 200 mmol·L-1 mannitol, 20% PEG and 200 mmol·L-1 NaCl stress. SL: Seedling leaf; MF: Meiotic floret; PL: Palea and lemma; P: Pistil; S: Stamen; FS: 5-day filling seeds"

Table 1

Species from genome database and their information"

Class Order Species Numbers
Eudicots Cucurbitales Cucumis sativus 5
Fabales Glycine max 16
Malvales Theobroma cacao 6
Myrtales Eucalyptus robusta 8
Brassicales Carica papaya 3
Ranunculales Aquilegia viridiflora 7
Vitales Vitis vinifera 7
Brassicales Arabidopsis thaliana 9
Malpighiales Populus trichocarpa 12
Lamiales Mimulus luteus 9
Solanales Solanum tuberosum 1
Monocots Poales Zea mays 14
Poales Brachypodium distachyon 10
Poales Oryza sativa 10
Poales Panicum virgatum 16
Poales Setaria italica 9
Poales Sorghum bicolor 10
ANA grade Amborellales Amborella trichopoda 7

Table 2

Species from transcriptome database and their in- formation"

Class Order Species Numbers
Eudicots Apiales Hedera nepalensis 9
Aquifoliales Ilex chinensis 8
Asterales Lactuca sativa 7
Celastrales Euonymus carnosus 9
Cornales Swida wilsoniana 9
Dipsacales Lonicera japonica 7
Ericales Camellia japonica 11
Fagales Cyclobalanopsis glauca 6
Cornales Aucuba japonica 8
Gentianales Vinca major 8
Proteales Platanus occidentalis 5
Oxalidales Oxalis corniculata 7
Proteales Meliosma arviflora 12
Sapindales Buxus sinica 6
Monocots Alismatales Acorus calamus 6
Asparagales Asparagus officinalis 4
Asparagales Phalaenopsis amabilis 6
Liliales Dioscorea opposita 8
Liliales Yucca smalliana 6
Pandanales Pandanus tectorius 5
Zingiberales Canna indica 8

Figure 6

The NJ/ML (neighbor joining/maximum likelihood) tree of MIP1 genes family in angiospermsThe MIP1 genes can be divided into six types (subfamilies) in angiosperm. Red: Monocotyledons; Purple: Eudicotyledons; Green: Amborella trichopoda. The numbers on each branch are bootstrap support values of NJ/ML (neighbor joining/maximum likelihood) trees. Asterisks stand for bootstrap of 100% and minus stand for bootstrap below 50%. The length of each branch is meaningless."

Figure 7

Simplified gene tree of type I MIP1 homologsRed: Dicotyledons; Purple: Monocotyledons"

Figure 8

Simplified gene tree of type V MIP1 homologsRed: Acorus calamus; Purple: Dicotyledons; Green: Amborella trichopoda"

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