Chin Bull Bot ›› 2017, Vol. 52 ›› Issue (2): 218-224.doi: 10.11983/CBB16033

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Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata

Man Chen, YishengTu*, Linan Ye, Biyun Yang   

  1. College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
  • Received:2016-02-24 Accepted:2016-09-04 Online:2017-04-05 Published:2017-03-01
  • Contact: YishengTu E-mail:ysttz2012@163.com
  • About author:

    # Co-first authors

Abstract:

We investigated the effects of amino acid supplements on thallus growth, superoxide dismutase (SOD) activity and huperzine-A accumulation in Huperzia serrata by adding aspartic acid, lysine, and tryptophan at different concentrations into the medium. With aspartic acid, the dry weight of the thallus increased by 18.50%-25.75% and the huperzine-A accumulation was significantly higher than in the control. The optimal concentration of aspartic acid was 0.5 mmol·L-1. The relative growth of thallus was significantly higher with 1 mmol·L-1 tryptophan than in the control, but the huperzine-A accumulation was slightly lower than for the control. The relative growth rate, dry weight and huperzine-A accumulation was lower for thallus cultured with lysine than the control. The SOD activity of the thallus cultured with aspartic acid was increased properly, and the huperzine-A accumulation of the thallus was increased. However, huperzine-A accumulation of the thallus was suppressed when SOD activity remained high in the long-term.

Figure 1

The HPLC (high performance liquid chromatography fluorescence) test of huperzine A standard (A) and extract of Huperzia serrata thallus in vitro culture (B)"

Figure 2

The thallus of Huperzia serrata supplemented with different amino acids(A) The thallus of H. serrata supplemented with Asp; (B) The thallus of H. serrata supplemented with Trp; (C) The thallus of H. serrata supplemented with Lys"

Table 1

Effect of different concentrations of Asp, Lys or Trp on the biomass of thallus in Huperzia serrata"

Concentration of different
amino acid
(mmol·L-1)
Relative
growth rate
(%)
Dry weight (g·L-1)
Water/control 1313.05±21 bc 7.61680
Asp 0.25 1189.68±19 bc 9.30778
Asp 0.50 1337.76±29 bc 9.02623
Asp 1.00 1217.20±25 bc 9.57869
Asp 2.00 1211.28±32 bc 7.92295
Lys 0.25 1108.18±27 c 7.57992
Lys 0.50 1222.61±31 bc 7.40164
Lys 1.00 1328.25±30 bc 7.58278
Lys 2.00 1231.90±35 bc 7.32459
Trp 0.25 1311.53±33 bc 7.80328
Trp 0.50 1460.42±24 ab 8.01024
Trp 1.00 1647.51±37 a 9.35860
Trp 2.00 1050.53±17 c 6.66844

Figure 3

Effect of different concentrations of Asp, Lys and Trp on the content of huperzine A in thallus of Huperzia serrata"

Figure 4

The change features of SOD activity for Huperzia serrata thallus supplemented with different amino acids(A) The change features of SOD activity for H. serrata thallus supplemented with 0.5 mmol·L-1 Asp and 0.25 mmol·L-1 Lys, respectively; (B) The SOD activity of H. serrata thallus supplemented with different concentrations of Asp on 50 days"

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