Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (2): 218-224.DOI: 10.11983/CBB16033

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Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata

Man Chen, YishengTu*, Linan Ye, Biyun Yang   

  1. College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
  • Received:2016-02-24 Accepted:2016-09-04 Online:2017-03-01 Published:2017-04-05
  • Contact: YishengTu
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    # Co-first authors


We investigated the effects of amino acid supplements on thallus growth, superoxide dismutase (SOD) activity and huperzine-A accumulation in Huperzia serrata by adding aspartic acid, lysine, and tryptophan at different concentrations into the medium. With aspartic acid, the dry weight of the thallus increased by 18.50%-25.75% and the huperzine-A accumulation was significantly higher than in the control. The optimal concentration of aspartic acid was 0.5 mmol·L-1. The relative growth of thallus was significantly higher with 1 mmol·L-1 tryptophan than in the control, but the huperzine-A accumulation was slightly lower than for the control. The relative growth rate, dry weight and huperzine-A accumulation was lower for thallus cultured with lysine than the control. The SOD activity of the thallus cultured with aspartic acid was increased properly, and the huperzine-A accumulation of the thallus was increased. However, huperzine-A accumulation of the thallus was suppressed when SOD activity remained high in the long-term.