Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (6): 807-816.DOI: 10.11983/CBB15222

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Somatic Embryo Induction and Plantlet Regeneration of Fraxinus velutina

Liping Yan1,2,3, Li Li1,2, Cuilan Liu1,2, Dejun Wu1,2*, Yinhua Wang1, Fei Ren1, Liangjun Zhao3   

  1. 1Shandong Provincial Academy of Forestry, Jinan 250014, China
    2Shandong Provincial Key Laboratory of Forest Tree Genetic Improvement, Jinan 250014, China
    3Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China
  • Received:2015-12-23 Accepted:2016-05-11 Online:2016-11-01 Published:2016-12-02
  • Contact: Wu Dejun
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    # Co-first authors

Abstract:

Using explants of Fraxinus velutina, we investigated the effects of basic medium, plant growth regulators, and concentrations of antioxidant medium, and culturing conditions on the induction of somatic embryogenesis in Fraxinus velutina. The best regeneration medium was Murashige and Skoog (MS) basal medium with Gamborg B5 vitamin supplementation, with 2.0 mg·L-1 6-benzyladenine (6-BA), 0.1 mg·L-1 naphthaleneacetic acid (NAA) at 20-day dark culture followed by light intensity of 100-120 μmol·m-2·s-1 irradiation for 14 h·d-1, and the culture temperature was (25±2)°C at daytime and (18±2)°C at night. More than 81.2% radicle segments produced adventitious shoots, with a mean number of adventitious shoots per explant of 9.3. The optimal growing medium was modified WPM medium supplemented with 0.5 mg·L-1 6-BA, 0.2 mg·L-1 ZT and 0.01 mg·L-1 NAA. More than 97.3% regeneration plantlets were able to root after transfer to modified half-strength MS medium supplemented with 1.0 mg·L-1 indole-3-butyric acid, 0.05 mg·L-1 NAA and 20 g·L-1 sucrose; 97.8% of the plantlets survived.