%A Liping Yan, Li Li, Cuilan Liu, Dejun Wu, Yinhua Wang, Fei Ren, Liangjun Zhao %T Somatic Embryo Induction and Plantlet Regeneration of Fraxinus velutina %0 Journal Article %D 2016 %J Chinese Bulletin of Botany %R 10.11983/CBB15222 %P 807-816 %V 51 %N 6 %U {https://www.chinbullbotany.com/CN/abstract/article_3134.shtml} %8 2016-11-01 %X

Using explants of Fraxinus velutina, we investigated the effects of basic medium, plant growth regulators, and concentrations of antioxidant medium, and culturing conditions on the induction of somatic embryogenesis in Fraxinus velutina. The best regeneration medium was Murashige and Skoog (MS) basal medium with Gamborg B5 vitamin supplementation, with 2.0 mg·L-1 6-benzyladenine (6-BA), 0.1 mg·L-1 naphthaleneacetic acid (NAA) at 20-day dark culture followed by light intensity of 100-120 μmol·m-2·s-1 irradiation for 14 h·d-1, and the culture temperature was (25±2)°C at daytime and (18±2)°C at night. More than 81.2% radicle segments produced adventitious shoots, with a mean number of adventitious shoots per explant of 9.3. The optimal growing medium was modified WPM medium supplemented with 0.5 mg·L-1 6-BA, 0.2 mg·L-1 ZT and 0.01 mg·L-1 NAA. More than 97.3% regeneration plantlets were able to root after transfer to modified half-strength MS medium supplemented with 1.0 mg·L-1 indole-3-butyric acid, 0.05 mg·L-1 NAA and 20 g·L-1 sucrose; 97.8% of the plantlets survived.