Chin Bull Bot ›› 2015, Vol. 50 ›› Issue (6): 768-778.doi: 10.11983/CBB14168

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Progress in Biosynthetic Pathways of Brassinosteroids

Hongyan Ren, Li Wang, Qingxiu Ma, Guang Wu*   

  1. College of Life Sciences, Shaanxi Normal University, Xi’an 710069, China
  • Received:2014-09-11 Accepted:2015-03-20 Online:2015-09-06 Published:2015-11-01
  • About author:

    ? These authors contributed equally to this paper

Abstract:

Brassinosteroids (BRs) play an important role in the progress of plant growth and development. This paper introduces the structure of BRs and how to study the biosynthetic pathway, then describes methods for biological and chemical detection. Finally, we detail the early C-6 oxidation pathway, the late C-6 oxidation pathway and the early C-22, C-23 hydroxylation pathway, their regulation and progress in the identification of BR-deficient mutants and enzymes for BR biosynthesis.

Figure 1

Structures of the brassinosteroids (BRs)"

Figure 2

Biosynthesis of campestanol (CN) from squalene-2,3-oxide (Zullo et al., 2002)"

Figure 3

Three biosynthetic pathways of brassinosteroids (BRs)"

Table 1

Brassinosteroid biosynthesis mutants"

植物 突变体 底物 基因的功能
拟南芥 dwf1 24-亚甲基胆甾醇 类固醇Δ24(28)-还原酶
dwf4 芸苔甾醇、(24R)-24-甲基-4-胆甾烷-3-酮、(24R)-24-甲基-5α-胆甾烷-3-酮、油菜烷醇和6-氧油菜烷醇 细胞色素P450 (CYP90B1)和C-22羟化酶
dwf5 5-脱氢麦角甾-7,24(24)-二烯醇 类固醇还原酶
dwf7 麦角甾-7,24(24)-二烯醇 类固醇还原酶
det2 (24R)-24-甲基-4-胆甾烷-3-酮和22-羟基-(24R)-24-甲基-4-胆甾烷-3-酮 类固醇-5α-还原酶
cpd 22-羟基芸苔甾醇、22,23-二羟基芸苔甾醇、6-脱氧长春花甾酮和6-脱氧茶甾酮 细胞色素P450 (CYP90A1)和C-3氧化酶
cyp90c1 22-羟基芸苔甾醇、22-羟基-(24R)-24-甲基-4-胆甾烷-3-酮、3-表-6-脱氧长春花甾酮和6-脱氧长春花甾酮 细胞色素P450 (CYP90C1)和C-23羟化酶
cyp90d1 22-羟基芸苔甾醇、22-羟基-(24R)-24-甲基-4-胆甾烷-3-酮、3-表-6-脱氧长春花甾酮和6-脱氧长春花甾酮 细胞色素P450 (CYP90D1)和C-23羟化酶
cyp85a1 6-脱氧茶甾酮、6-脱氧香蒲甾醇、3-脱氢-6-脱氧茶甾酮和6-脱氧油菜素甾酮 细胞色素P450 (CYP85A1)和C-6氧化酶
cyp85a2 6-脱氧茶甾酮、6-脱氧香蒲甾醇、3-脱氢-6-脱氧茶甾酮、6-脱氧油菜素甾酮和油菜素甾酮 细胞色素P450 (CYP85A2)和C-6氧化酶, 将CS氧化为BL
豌豆 lkb 24-亚甲基胆甾醇 类固醇Δ24(28)-还原酶
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