植物学报 ›› 2019, Vol. 54 ›› Issue (3): 335-342.DOI: 10.11983/CBB18216

• 研究报告 • 上一篇    下一篇

不同产地姜黄属植物中姜黄素类成分及矿质元素分析

杨小芳1,陈育才2,刘建福1,*(),李丹丹1,胡娴1   

  1. 1. 华侨大学园艺系, 厦门 361021
    2. 南安市丰州镇农业服务中心, 泉州 362330
  • 收稿日期:2018-10-17 接受日期:2019-02-20 出版日期:2019-07-01 发布日期:2019-11-24
  • 通讯作者: 刘建福
  • 基金资助:
    福建省产业技术联合创新项目(闽发改高技[2014]514号和泉州市科技计划项目No.2018N001)

Analysis of Curcuminoids and Mineral Elements in Curcuma from Different Habitats

Xiaofang Yang1,Yucai Chen2,Jianfu Liu1,*(),Dandan Li1,Xian Hu1   

  1. 1. Department of Horticulture, Huaqiao University, Xiamen 361021, China
    2. Agricultural Service Center of Fengzhou Town Nan’ an City, Quanzhou 362330, China;
  • Received:2018-10-17 Accepted:2019-02-20 Online:2019-07-01 Published:2019-11-24
  • Contact: Jianfu Liu

摘要: 以福建泉州地区种植的12个姜黄(Curcuma longa)和莪术(Curcuma zedoaria)种质的成熟根茎为试材, 采用正交设计法优化姜黄素(CCM)、单脱甲氧基姜黄素(DMC)和双脱甲氧基姜黄素(BDMC)的提取工艺条件, 比较不同产地姜黄属种质的3种姜黄素类化合物和16种矿质元素的含量。结果表明, 姜黄素类化合物提取工艺为: 100%甲醇, 1:5 (g·mL-1)固液比, 超声提取2分钟, 提取率达到最高值。CCM、DMC和BDMC的最大吸收波长分别为424、418及414 nm; 回归方程分别为Y=0.170 3X+0.024 3 (CCM), Y=0.173 8X+0.041 (DMC), Y=0.140 6X+0.051 4 (BDMC)。GY03种质中CCM、DMC和BDMC含量分别为1.23%、1.22%与1.50%, 总姜黄素类含量最高, 达3.95%; GY01种质的总姜黄素次之(为3.82%); GY03和GY01可在栽培地推广种植; 12个姜黄属种质的16种矿质元素中, 以Mg、Mn、Al、Fe和Zn含量较为丰富。

关键词: 姜黄, 莪术, 姜黄素类化合物, 矿质元素, ICP-MS

Abstract: In this study, the mature rhizomes of 12 germplasms of Curcuma longa and C. zedoaria from different origins cultivated in Fujian, Quanzhou, were used as experimental materials. The orthogonal design method was used to optimize the extraction of curcumin (CCM), demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), and the content of 3 curcuminoids and 16 mineral elements in different germplasms were compared. The extraction rate peaked with the following extraction process: 100% methanol as solvent, 1:5 (g·mL-1) solid-liquid ratio, and ultrasonic extraction for 2 min. The maximum absorption wavelengths for CCM, DMC and BDMC were 424, 418 and 414 nm, respectively. The linear equations for the 3 curcuminoids were Y=0.170 3X+0.024 3 (CCM), Y=0.173 8X+0.041 (DMC), and Y=0.140 6X+0.051 4 (BDMC). The content of CCM, DMC and BDMC in GY03 was 1.23%, 1.22% and 1.50%, respectively, and that of total curcumin was the highest, 3.95%; The content of total curcumin in GY01 was next, up to 3.82%. GY03 and GY01 can be promoted in cultivated land as excellent breeding material. Among the 16 mineral elements detected in 12 turmerics, the contents of Mg, Mn, Al, Fe, and Zn were relatively abundant.

Key words: Curcuma longa, C. zedoaria, curcuminoids, mineral elements, ICP-MS