植物学报 ›› 2013, Vol. 48 ›› Issue (6): 651-657.doi: 10.3724/SP.J.1259.2013.00651

• 技术方法 • 上一篇    下一篇

仙鹤藓的孢子萌发及愈伤组织诱导

吴小凤, 胡若洋*, 李学东   

  1. 首都师范大学生命科学学院, 北京 100048
  • 收稿日期:2012-09-12 修回日期:2012-12-13 出版日期:2013-11-01 发布日期:2013-12-03
  • 通讯作者: 胡若洋 E-mail:huruoyang@aliyun.com

Spore Germination and Callus Induction of Atrichum undulatum

Xiaofeng Wu, Ruoyang Hu*, Xuedong Li   

  1. School of Life Sciences, Capital Normal University, Beijing 100048, China
  • Received:2012-09-12 Revised:2012-12-13 Online:2013-11-01 Published:2013-12-03
  • Contact: Ruoyang Hu E-mail:huruoyang@aliyun.com

摘要: 通过对采自河北雾灵山海拔1 500 m的仙鹤藓(Atrichum undulatum)的孢子萌发以及原丝体发育的观察, 发现仙鹤藓孢子无休眠现象, 孢子接种3天左右萌发; 其原丝体发育分为绿丝体和轴丝体两个阶段。扩大培养实验结果表明, 仙鹤藓茎叶体在添加2%葡萄糖的MS培养基上, 置于25°C/20°C、14小时光照/10小时黑暗、36 μmol·m–2·s–1条件下培养, 产生新生茎叶体最多, 且茎叶体长势最好, 可以获得大量无菌材料。仙鹤藓愈伤组织诱导实验显示, 形成愈伤组织的最佳培养基为添加2%葡萄糖和1.0 mg·L–1 6-BA的MS培养基。

Abstract: We studied the spore germination and protonema development of Atrichum undulatum collected from Wuling Mountain, Hebei Province (1 500 m a.s.l.). The dormancy stage is absent during spore development. Spores in incubation culture take 3 days to germinate. Spores germinate exogenously by forming a protonema in 2 growth phases: chloronema and caulonema. The best growth conditions were MS medium with 2% glucose at 25°C/20°C, 14 h light/10 h dark, 36 μmol·m–2·s–1. The optimal medium for callus induction is MS medium supplemented with 2% glucose and 1.0 mg?L–1 6-BA to obtain high-quality cormus for large-scale cultivation.

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