植物学报 ›› 2011, Vol. 46 ›› Issue (2): 179-188.doi: 10.3724/SP.J.1259.2011.00179

• 研究报告 • 上一篇    下一篇

东海剧毒卡尔藻的形态特征及其系统进化分析

王红霞, 陆斗定*, 黄海燕, 戴鑫烽, 夏平   

  1. 国家海洋局第二海洋研究所, 国家海洋局海洋生态系统与生物地球化学重点实验室, 杭州 310012
  • 收稿日期:2010-07-23 修回日期:2010-11-07 出版日期:2011-03-01 发布日期:2011-05-09
  • 通讯作者: 陆斗定 E-mail:doudinglu@126.com
  • 基金资助:

    863计划;973计划;公益行业项目;908专项资助项目

Morphological and Phylogenetic Analysis of Karlodinium veneficum Isolated from the East China Sea in China

Hongxia Wang, Douding Lu*, Haiyan Huang, Xinfeng Dai, Ping Xia   

  1. Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China
  • Received:2010-07-23 Revised:2010-11-07 Online:2011-03-01 Published:2011-05-09
  • Contact: Dou-Ding LU E-mail:doudinglu@126.com

摘要: 利用光学显微镜、荧光显微镜、扫描电镜及分子生物学等方法, 对分布于我国东海海域的剧毒卡尔藻(Karlodinium veneficum)藻株(LAMB090611)的形态特征和显微结构进行了描述, 并探讨了其分子系统进化关系。该藻株细胞长11.1–18.7 μm, 平均值为(14.2±1.8) μm, 宽8.2–14.7 μm, 平均值为(10.8±1.5) μm。细胞形态结构特征为: 上下锥体积基本相同; 顶沟短而直; 腹孔明显; 纵沟延伸至上锥; 横沟错位距离约占细胞总长的28%–38%; 含有2或4个不规则形态的叶绿体; 细胞核位于中部或下锥。此藻种的暴发可引发有害赤潮(harmful algal bloom)。当前加强有害赤潮的预防和监测工作是减少危害的有效途径, 而对引发赤潮原因种的准确识别和鉴定则是基础和关键。

Abstract: Karlodinium veneficum (D. Ballantine) J. Larsen (LAMB090611), isolated from the East China Sea of Zhejiang province, was reported for the first time in China. The strain of LAMB090611 was described on the basis of morphological characteristics, and molecular phylogenetics. The results of this study were acquired by light microscopy, fluorescence microscopy, scanning electron microscopy and molecular sequencing. The cells have a mean length of 14.2±1.8 μm (range 11.1–18.7 μm) and mean width of 10.8±1.5 μm (range 8.2–14.7 μm). The size of epicone is approximately the same as that of hypocone. The cell has a short, straight apical groove and distinct ventral pore, with usually 2 or 4 chloroplasts distributed irregularly in the cell. The sulcus extends onto the epicone. The cingulum displacement is about 28%–38% of the body length. The nucleus is in middle of the cell or in the hypocone. A high density of this species results in harmful algal bloom. Therefore, the precise identification and detection of the causative species are important for monitoring and mitigation of such blooms.

中图分类号: 

  • Q

[1].参考文献
[2]侯建军, 赖红艳, 黄邦欣.用PCR扩增裸甲藻和微小原甲藻rRNA基因的方法研究.[J].湖北民族学院学报,2005,23:82-85
[3]陆斗定, 齐雨藻, Jeanette Goebel, 邹景忠, 高亚辉 . 东海原甲藻修订及与相关原甲藻的分类学比较. [J].应用生态学报,2003,14:1060-1064
[4]苏纪兰 .中国的赤潮研究.[J].中国科学院院刊,2001,16:339-342
[5]张传松, 王江涛, 朱德弟, 王修林, 李京 . 2005年春夏季东海赤潮过程中营养盐作用初探. [J].海洋学报,2008,30:153-159
[6]周名江, 朱明远, 张经 . 中国赤潮的发生趋势和研究进展. [J].生命科学,2001,13:54-59
[7]Adolf JE, Bachvaroff TR, Place AR.Environmental modulation of karlotoxin levels in strains of the cosmopolitan dinoflagellate Karlodinium veneficum (Dinophyceae)[J].J. Phycol,2009,45:176-192
[8]Ajani P, Hallegraeff G., Pritchard T.Historic overview of algal blooms in marine and estuarine waters of New South Wales, Australia[J].Proc. Linn. Soc. N. S. W,2001,123:1-22
[9]Anderson DM, Garrison DJ . Ecology and oceanography of harmful algal blooms. [J].Limnol. Oceanogr,1997,42:1009-1305
[10]Bachvaroff TR, Adolf JE, Place AR.Strain variation in Karlodnium veneficum (Dinophyceae): toxin profiles, pigments, and growth characteristics[J].J. Phycol,2009,45:137-153
[11]Ballantine D.Two new marine species of Gymnodinium isolated from the Plymouth area[J].J. Mar. Biolog. Assoc. U.K,1956,35:467-474
[12]Bergholtz T, Daugbjerg N, Moestrup ?, Fernandez-Tejedor M.On the identity of Karlodinium veneficum and description of Karlodinium arminger spnov. (Dinophyceae), based on light and electron microscopy, nuclear-encoded LSU rDNA, and pigment composition.[J].J. Phycol,2005,42:170-193
[13]Bjornland T, Tangen K.Pigmentation and morphology of a marine Gyrodinium (Dinophyceae) with a major carotenoid different from peridinin and fucoxanthin[J].J. Phycol,1979,15:457-463
[14]Bolch CJS, Negri AP, Hallegraeff GM.Gymnodinium microreticulatum sp.nov. (Dinophyceae): a naked, microreticulate cyst-producing dinoflagellate, distinct from Gymnodinium catenatum and Gymnodinium nolleri.[J].Phycologia,1999,38:301-313
[15]Braarud T.A red water organism from Walvis Bay(Gymnodinium galatheanumn)[J].Galathea Report,1957,1:137-138
[16]Cosgrove J, Grigo S, Hosja W, Hallegraeff G . The investigation of a dinoflagellate associated with a fish kill event in the Murray River/estuary, Western Australia. In Hallegraeff GM, Blackburn SI, Bolch CJ, Lewis R J, Eds. [J].Abstract in 9th International Conference on Harmful Algal Bloom.,2000,:518-
[17]Daugbjerg N, Hansen G., Larsen J, Moestrup ?.Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmoured dinoflagellates[J].Phycologia,2000,39:302-317
[18]Deeds JR, Terlizzi DE, Adolf JE, Stoecker DK, Place AR.Toxic activity from cultures of Karlodinium micrum (=Gyrodinium galatheanum) (Dinophyceae) a dinoflagellate associated with fish mortalities in an estuarine aquaculture facility[J].Harmful Algae,2002,1:169-189
[19]Deeds JR, Place AR.Sterol specific membrane interactions with the toxins from Karlodinium micrum (Dinophyceae)— a strategy for self-protection[J].Afr. J. Mar. Sci,2006,28:421-425
[20]De Salas MF, Bolch CJS, Botes L, Nash G, Wright SW, Hallegraeff GM.Takayama gen.nov. (Gymnodiniales, Dinophyceae), a new genus of unarmored dinoflagellates with sigmoid apical grooves, including the description of two new species.[J].J. Phycol,2003,39:1233-1246
[21]De Salas MF, Bolch CJS, Hallegraeff GM.Karlodinium australe sp.nov. (Gymnodiniales, Dinophyceae), a new potentially ichthyotoxic unarmoured dinoflagellate from lagoonal habitats of south-eastern Australia.[J].Phycologia,2005,44:640-650
[22]De Salas MF.Novel unarmored dinoflagellates from the toxigenic family Karrniaceae (Gymnodiniales): five new species of Karlodinium and one new Takayama from the Australian sector of the southern ocean[J].J. Phycol,2008,44:241-257
[23]Fensin EE .Occurrence and ecology of the dinoflagellate Karlodinium micrum in estuaries of North Carolina, USA.. [J].Harmful Algae 2002 Proceedings,2004,:62-64
[24]Garces E, Fernandez M, Penna A, Lenning KV, Gutierrez A, Camp J, Zapata M.Characterization of NW Mediterranean Karlodinium spp(Dinophyceae) strains using morphological, molecular, chemical, and physiological methodologies. [J].J Phycol,2006,42:1096-1112
[25]Goshorn D, Deeds J, Tango P, Poukish C, Place AR, McGinty M, Butler W, Luckett C, Magnien R. Occurrence of Karlodinium micrum and its association with fish kills in Maryland estuaries.[J].Harmful Algae 2002 Proceeding,2004,:361-363
[26]Hallegraeff GM . A review of harmful algal blooms and their apparent global increase.[J].Phycologia,1993,32:79-99
[27]Hallegraeff GM , Anderson DM and Cembella AD eds . Manual on Harmful Marine Microalgae. [J].IOC Manuals Guides No. 33.,1995,:-
[28]Hou JJ, Lai HY, Huang BQ.Comparative study on method of amplifying the rRNA of the genomes from Gymnodinium spand Prorocentrum minium by PCR.[J].Journal of Hubei Institute for Nationalities Natural Science Edition,2005,23:82-85
[29]Kempton JW, Lewitus AJ, Deeds JR, Law JM, Place AR.Toxicity of Karlodinium micrum (Dinophyceae) associated with a fish kill in a South Carolina brackish retention pond[J].Harmful Algae,2002,1:233-241
[30]Leadbeater B, Dodge JD.The fine structure of Woloszynskia micra sp[J].nov., a new marine dinoflagellate. British Phycological Journal,1966,3:1-17
[31]Li AS, Stoecker DK, Coats DW .Mixotrophy in Gyrodinium galatheanum (Dinophyceae): grazing responses to light intensity and inorganic nutrients.[J].J. Phycol.,2000, 36:33-45
[32]Nielsen MV.Growth and chemical composition of the toxic dinoflagellate Gymnodinium galatheanum in relation to irradiance, temperature and salinity[J].Mar. Ecol. Prog,1996,Ser.136:205-211
[33]Pieterse F, Van Der Post DC.The pilchard of SouthWest Africa Oceanographical conditions associated with red tides and fish mortalities in the Walvis Bay region.[J].Invest. Rep. Mar. Res. Lab. S.W. Afr,1967,14:1-125
[34]Place A ). Amphidinols and karlotoxins—brothers in arms. [J].In Abstracts from XI Int. Conf. Harmful Algal Blooms.,2004,:211-
[35]Schiller J . Dinoflagellatae.[J].In: Raben-horsts Kryptogamenflora,1933,10:1-617
[36]Terlizzi DE, Stoecker DK, Glibert PM .Gyrodinium galatheanum: a threat to estuarine aquaculture. responsible aquaculture in the new millenium. [J].Abstracts of Contributions Presented at the International Conference AQUA,2000,:700-
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