Salt-alkali tolerant germplasm identification in faba bean lays the foundation for the exploration of salt-alkali tolerant genes and for the selection and breeding of salt-alkali tolerant varieties, which is of great significance for the utilization of salinized land. In this study, 400 faba bean accessions from domestic and foreign collections were subjected to stress treatment with 8 gL-1 mixed salt-alkali (NaCl, Na2CO3 and Na2SO4, pH=9.25) at germination stage. Three germination indexes such as germination rate and 13 root indexes such as root length were measured. Correlation, principal component, membership function and systematic cluster analysis were used to comprehensively evaluate and classify the salt-alkali tolerance of various accessions. The results showed that: (1) Under salt-alkali stress, overlap number was most affected, followed by the coila, and the average diameter of root was stable. (2) The number of root tips and the number of endpoints and bifurcation, the total length of root and colia and connections, the number of ends and bifurcation, colia and connections and bifurcation, and the number of connections and bifurcation, were positively correlated (P<0.01). While the total number of root and the total length of root, total projected area of root, total surface area of root, total volume of root, coila, number of connection, bifurcation number, number of overlap and total number of connection, the average diameter of roots and the number of root tip, total length of root, endpoint, coila, number of connection, number of fork, number of overlap and total number of connection, were negatively correlated (P<0.01). (3) Root total surface area, root total projected area, root total length and root total volum, can be used as the key indexes to identify the salt-alkali tolerance of faba bean at germination stage. (4) Two salt-alkali tolerant accessions H0001857 and H0002338, and three salt-alkali intolerant accessions H0001819, H0000647 and H0000971, were selected, respectively. (5) 400 faba bean accessions were divided into 4 group: group I was salt-alkali tolerance accessions, accounting for 4.5%; Group II was moderately salt-alkali tolerant accessions, constituting 19.5% of the total; Group III was weakly saline-tolerant, comprising 42.9% of the tested accessions; Group IV was salt-alkali intolerant accessions, constituting 33% of the total.The salt-alkali tolerance of faba bean was identified and comprehensively evaluated on root phenotypic and germination traits. Variation and correlation of each index, the key indexes of salt-alkali tolerance identification were identified. Extreme materials could be used for the study of salt-alkali tolerance mechanisms in faba bean, the exploration of salt-alkali tolerant genes, and the selection and breeding of salt-alkali tolerant varieties.
Huiling Fan, Yan Lu, Wenhai Jin, Hui Wang, Xiaoxing Peng, Xuexia Wu, Yujiao Liu
. Identification and Comprehensive Evaluation of Faba Bean Salt-Alkali Tolerance Based on Root Phenotypic Traits at Germination Stage[J]. Chinese Bulletin of Botany, 0
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DOI: 10.11983/CBB24093
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