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Chinese Bulletin of Botany >

2025 , Vol. 60 >Issue 1: 1 - 0

DOI: https://doi.org/10.11983/CBB24068

Cloning and Functional Analysis of BnaA02CPSF6 gene from Brassica napus L.

  • LI Jing-Xiang ,
  • LIU Cui ,
  • HE Li ,
  • PENG Pan ,
  • MA Jia-Jin ,
  • HU Zi-Yi ,
  • LIU Hong-Bei
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  • College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China

Received date: 2024-05-05

  Revised date: 2024-07-19

  Online published: 2024-07-29

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Abstract

The CPSF family (cleavage and polyadenylation specificity factor) is a crucial protein factor that is responsible for polyadenylation signal recognition in mRNA precursors, cleavage and the addition of poly(A) tails to mRNA in plants, it plays a crucial role in the regulation of flowering time, environmental response, and seed development. Currently, the function of the CPSF family in Brassica napus is unclear. To explore the function and expression patterns of the CPSF gene family, this study cloned BnaA02.CPSF6 from B. napus variety 'Zhongshuang 11' and conducted bioinformatics analysis, subcellular localization, expression pattern analysis, and functional characterization of the gene. The results indicate that the coding region of the BnaA02.CPSF6 gene is 1938 bp, encoding 646 amino acids without intron structures. Its promoter region contains multiple cis-acting elements involved in light responses and MYB binding sites. Additionally, there are six homologous genes of BnaA02.CPSF6 in B. napus. The BnaA02.CPSF6 gene is expressed in root, stem, leaf, flower and different developmental seed of B. napus, especially significantly higher in 15-35d developmental seed, and its encoded protein is localized in the nucleus. The BnaA02.CPSF6 gene expression is upregulated under salt and drought stress. Under the treatment of hormones such as ABA, IAA, GA, SA, and MeJA, the expression of the BnaA02.CPSF6 gene is initially inhibited and then gradually recovers to normal levels. Under normal condition, overexpression of the BnaA02.CPSF6 gene in Arabidopsis thaliana results in an early bolting and flowering phenotype, along with a reduced number of rosette leaves. In summary, the above results indicate that the BnaA02.CPSF6 is involved in abiotic stress responses and regulated by phytohormones, may also play a promoting role in flowering regulation.

Key words： Brassica napus; BnaA02CPSF6; Gene function; Expression patterns; Growth and development

Cite this article

LI Jing-Xiang , LIU Cui , HE Li , PENG Pan , MA Jia-Jin , HU Zi-Yi , LIU Hong-Bei . Cloning and Functional Analysis of BnaA02CPSF6 gene from Brassica napus L.[J]. Chinese Bulletin of Botany, 2025 , 60(1) : 1 -0 . DOI: 10.11983/CBB24068

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