Establishment of an In Vitro Regeneration System for Stem Segments of Cultivated Dioscorea polystachya
- School of Life Sciences, Fudan University, Shanghai 200438, China
Received date: 2024-04-08
Accepted date: 2024-05-27
Online published: 2024-05-27
Abstract
To establish a tissue culture regeneration system for cultivated Chinese yam (Dioscorea polystachya), the effects of different concentrations of plant growth regulators, and stem segments on axillary bud induction and plant regeneration were investigated with different concentrations of plant growth regulators, media types, and shoot tips and stem segments at four different positions under the shoot tip as explants. The results revealed that MS+1.0 mg∙L-1 6-BA+0.5 mg∙L-1 KT hormone ratio and 12-20 cm stem segment under the stem tip were the best formulas for the induction of adventitious buds in D. polystachya, and the induction rate reached 90.0%. In the subculture, the optimal concentration was MS+0.5 mg∙L-1 6-BA+0.05 mg∙L-1 NAA+0.1 mg∙L-1 KT, and the proliferation coefficient increased with increasing of 6-BA within a certain range when the low concentration of NAA was constant. With the same concentration of exogenous hormones, DKW medium was significantly better than the conventional 1/2MS medium, and the rooting rate is significantly improved, reaching 92.86%. Adding 1 mg PVP (polyvinylpyrrolidone) and increasing the number of transfers can significantly reduce browning. This study effectively solved the problem of optimizing the explant position for the stable of in vitro propagation of cultivated yam germplasm resources, laying a good foundation for the large-scale production of their high-quality virus-free seedlings.
Key words： Dioscorea polystachya; stem segments; tissue culture; in vivo regeneration
Cite this article
Wen Feng , Yuguo Wang . Establishment of an In Vitro Regeneration System for Stem Segments of Cultivated Dioscorea polystachya[J]. Chinese Bulletin of Botany, 2024 , 59(5) : 792 -799 . DOI: 10.11983/CBB24051
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