Phosphorus is one of the indispensable nutrients for plant growth and development. Orthophosphate (P) content in the soil is particularly rich, but due to the fixation of the soil, the effective phosphorus content that can be absorbed and utilized by plants is not high. Therefore improving the absorption and utilization capacity of plants to soil phosphorus, or optimizing the application of phosphate fertilizer has become an urgent problem that needs to be solved. The content of phosphite (PH) in soil is second only after orthophosphate, which has the characteristics of higher solubili- ty, two-way transport between plant xylem and phloem, but is not easy to be fixed by soil. Research on phosphite as phosphate fertilizer to replace orthophosphate fertilizer and phosphite-tolerant crop varieties breeding has rarely been reported. Therefore, in this study, five introduced potato (Solanum tuberosum) genotypes and one commercial variety Qingshu No.9 (QS9) were selected as research materials, and the seedlings were directly planted into the test field after domestication and refining, and normal phosphate fertilizer treatment and phosphite substitution treatment were set up to determine the phenotype, photosynthesis, dry matter and other indicators of different genotypes. Moreover, the phosphite tolerant coefficient (PTC) of each indicator was used as the measurement basis. Comprehensive evaluation of the PH resistance of different potato genotypes was conducted based on principal component analysis and other methods. The obtained results showed that the six potato varieties can be classified into three types: highly phosphite-tolerant (C115, and D13), weakly phosphite-tolerant (C20, C31, and QS9) and phosphite-sensitive (C80). This study evaluated the tole- rance of different potato genotypes to phosphite, thus providing scientific basis for the selection of phosphite-tolerant varieties and the development of new phosphite fertilizers.