Cloning and Functional Verification of the Borneol Dehydrogenase Encoding Gene AArBDH1 in Artemisia argyi
Received date: 2022-06-17
Accepted date: 2022-11-15
Online published: 2022-11-15
Borneol is one of the most important pharmacodynamic components in Artemisia argyi, which has antibacterial, anti-inflammatory, analgesic and other pharmacological activities. The synthesis and metabolism of borneol are affected by many kinds of enzymes, and borneol dehydrogenase is one of the key enzymes that oxidize borneol to camphor. In this study, the contents of borneol and camphor in 35 germplasm of A. argyi leaves were determined by Gas chromatograph-mass spectrometer (GC-MS). We found that the amount of borneol and camphor was highly varied among the varieties, and some germplasm contained more camphor than borneol, indicating that a large proportion of borneol was oxidized to camphor in A. argyi leaves. Based on the full-length transcriptome of A. argyi and homology comparison analysis, we cloned the first borneol dehydrogenase encoding gene AArBDH1 in A. argyi. The AArBDH1 contained 2 exons and 1 intron, and encoded a protein of 289 amino acids. The AArBDH1 gene expression levels were measured by real-time quantitative reverse transcription PCR (qRT-PCR) technology, which showed that AArBDH1 was differentially expressed in different tissues and in leaves at different development stages, and highly expressed in stem and 30 days old leaves. The enzymatic reactions of AArBDH1 with borneol as substrate and NAD+ as coenzyme showed that AArBDH1 could catalyze the dehydrogenation of borneol to camphor. Our study provides a theoretical basis and gene resource for further analyzing the regulation and potential improvement of borneol accumulation in A. argyi leaves.
Key words： Artemisia argyi; borneol; borneol dehydrogenase; camphor
Changjie Chen, Yuhuan Miao, Dandan Luo, Zixin Wang, Lujuan Guo, Tingting Zhao, Dahui Liu . Cloning and Functional Verification of the Borneol Dehydrogenase Encoding Gene AArBDH1 in Artemisia argyi[J]. Chinese Bulletin of Botany, 2023 , 58(4) : 560 -572 . DOI: 10.11983/CBB22123
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