Characteristics and Expression Specificity of <i>RCA</i> Genes in Two Ecotypes of <i>Phragmites australis</i>

Qiu Tianhang, Wang An’an, Li Li, Wang Yingchun, Cui Jipeng, Wang Ziyao, Wang Rui, Cui Suxia

doi:10.11983/CBB22204

Chinese Bulletin of Botany >

2023 , Vol. 58 >Issue 5: 687 - 700

DOI: https://doi.org/10.11983/CBB22204

EXPERIMENTAL COMMUNICATIONS

Characteristics and Expression Specificity of RCA Genes in Two Ecotypes of Phragmites australis

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Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China

Received date: 2022-08-31

Accepted date: 2022-10-24

Online published: 2022-10-25

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Abstract

(reed) is a cosmopolitan species, with huge biomass and high environmental adaptability. Restricted by its polyploidy genome, it is very difficult to study its unique properties at the genetic level. Using third-generation sequencing and multiple methods, we comprehensively studied the type, structure, expression, and localization patterns of RCA genes (encoding Rubisco activating enzyme) in two reed ecotypes (2n=8x). There are four types of RCA genes in Phragmites genome, all belonging to the RCA2β category. At the transcriptional and protein levels, we adopted immunogold method to detect the localization of RCA proteins. We found that RCAs have obvious ecotype specificity. Compared with swamp reeds (SR), desert-dune reeds (DR) have low RCA expression and RCA2-β2 are preferentially expressed. Six highly expressed RCA isoforms were identified though two-dimensional electrophoresis (2-DE) and mass spectrometry (MS), and were distributed in a high proportion in membrane fraction in DR. This result supports the hypothesis that RCA may transfer to the membrane area as the environment deteriorates, thereby giving RCA a protective function on the chloroplast membrane.

Key words： Phragmites australis; octoploid; desert ecotype; RCA; membrane localization

Cite this article

Qiu Tianhang, Wang An’an, Li Li, Wang Yingchun, Cui Jipeng, Wang Ziyao, Wang Rui, Cui Suxia . Characteristics and Expression Specificity of RCA Genes in Two Ecotypes of Phragmites australis[J]. Chinese Bulletin of Botany, 2023 , 58(5) : 687 -700 . DOI: 10.11983/CBB22204

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