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Effect of Nitrogen-doped Graphene Quantum Dots on Growth Direction of Primary Root in Arabidopsis thaliana

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  • Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response in Shanxi Province, School of Life Science, Shanxi Normal University, Taiyuan 030031, China
*E-mail: hhwrsl@163.com

Received date: 2022-03-14

  Accepted date: 2022-06-28

  Online published: 2022-06-28

Abstract

Graphene quantum dots (GQDs) have substantial application potentials in various fields such as electrochemical biosensor, bioimaging and biomedicine. Therefore, with the increasing exposure to the public and environment, their biosafety has aroused increasing concerns in recent years. So far, the influence of GQDs on the growth and development of plants is still poorly understood. In this study, we investigated the influence of nitrogen-doped GQDs (N-GQDs) treatment on the growth direction of primary root in Arabidopsis thaliana at cellular and molecular levels. We found that the N-GQDs were absorbed by roots and transported via vascular bundles. After the N-GQDs treatment at the concentration of 50-100 mg∙L-1, the growth direction of primary roots was changed, curving towards the outside of the culture medium. Because of the N-GQDs treatment, the starch granule accumulation of columnar cells was reduced, the abundance of auxin efflux carrier PIN3 was repressed, and the PIN3 in columnar cells was relocated to outer lateral membrane distant from the culture medium (towards the air), which resulted in the asymmetric auxin distribution in the root tips and the curved growth of primary roots towards a direction distant from the medium in order to run away from the high-concentration N-GQDs environment. The study results provide a reference direction for exploring the possible coping mechanism of plants with N-GQDs treatment, and also provide corresponding data for biosafety evaluation of N-GQDs.

Cite this article

Ye Qing, Yan Xiaoyan, Chen Huize, Feng Jinlin, Han Rong . Effect of Nitrogen-doped Graphene Quantum Dots on Growth Direction of Primary Root in Arabidopsis thaliana[J]. Chinese Bulletin of Botany, 2022 , 57(5) : 623 -634 . DOI: 10.11983/CBB22048

References

[1] 高坤, 常金科, 黎家 (2018). 植物根向水性反应研究进展. 植物学报 53, 154-163.
[2] 韩雯, 韩榕 (2015). 不同时间的UV-B辐射对拟南芥幼苗生长的影响. 植物学报 50, 40-46.
[3] 李晓阳, 陈慧泽, 韩榕 (2013). UV-B辐射对拟南芥种子萌发和幼苗生长的影响. 植物学报 48, 52-58.
[4] Baldwin KL, Strohm AK, Masson PH (2013). Gravity sensing and signal transduction in vascular plant primary roots. Am J Bot 100, 126-142.
[5] Band LR, Wells DM, Larrieu A, Sun JY, Middleton AM, French AP, Brunoud G, Sato EM, Wilson MH, Péret B, Oliva M, Swarup R, Sairanen I, Parry G, Ljung K, Beeckman T, Garibaldi JM, Estelle M, Owen MR, Vissenberg K, Hodgman TC, Pridmore TP, King JR, Vernoux T, Bennett MJ (2012). Root gravitropism is regulated by a transient lateral auxin gradient controlled by a tipping-point mechanism. Proc Natl Acad Sci USA 109, 4668-4673.
[6] Blancaflor EB, Fasano JM, Gilroy S (1998). Mapping the functional roles of cap cells in the response of Arabidopsis primary roots to gravity. Plant Physiol 116, 213-222.
[7] Brunoud G, Wells DM, Oliva M, Larrieu A, Mirabet V, Burrow AH, Beeckman T, Kepinski S, Traas J, Bennett MJ, Vernoux T (2012). A novel sensor to map auxin response and distribution at high spatio-temporal resolution. Nature 482, 103-106.
[8] Caspar T, Pickard BG (1989). Gravitropism in a starchless mutant of Arabidopsis: implications for the starch-statolith theory of gravity sensing. Planta 177, 185-197.
[9] Chakravarty D, Erande MB, Late DJ (2015). Graphene quantum dots as enhanced plant growth regulators: effects on coriander and garlic plants. J Sci Food Agric 95, 2772-2778.
[10] Deng S, Jia PP, Zhang JH, Junaid M, Niu AP, Ma YB, Fu AL, Pei DS (2018). Transcriptomic response and perturbation of toxicity pathways in zebrafish larvae after exposure to graphene quantum dots (GQDs). J Hazard Mater 357, 146-158.
[11] Ding Y, Cheng HH, Zhou C, Fan YQ, Zhu J, Shao HB, Qu LT (2012). Functional microspheres of graphene quantum dots. Nanotechnology 23, 255605.
[12] Feng P, Geng BJ, Cheng Z, Liao XY, Pan DY, Huang JY (2019). Graphene quantum dots-induced physiological and biochemical responses in mung bean and tomato seedlings. Braz J Bot 42, 29-41.
[13] Friml J, Wi?niewska J, Benková E, Mendgen K, Palme K (2002). Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis. Nature 415, 806-809.
[14] Galvan-Ampudia CS, Julkowska MM, Darwish E, Gandullo J, Korver RA, Brunoud G, Haring MA, Munnik T, Vernoux T, Testerink C (2013). Halotropism is a response of plant roots to avoid a saline environment. Curr Biol 23, 2044-2050.
[15] Galvan-Ampudia CS, Testerink C (2011). Salt stress signals shape the plant root. Curr Opin Plant Biol 14, 296-302.
[16] Grunewald W, Friml J (2010). The march of the PINs: developmental plasticity by dynamic polar targeting in plant cells. EMBO J 29, 2700-2714.
[17] Guo XQ, Mei N (2014). Assessment of the toxic potential of graphene family nanomaterials. J Food Drug Anal 22, 105-115.
[18] Hasanuzzaman M, Nahar K, Alam M, Roychowdhury R, Fujita M (2013). Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. Int J Mol Sci 14, 9643-9684.
[19] Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000). Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 51, 463-499.
[20] Hu XG, Zhou QX (2013). Health and ecosystem risks of graphene. Chem Rev 113, 3815-3835.
[21] Ju J, Chen W (2014). Synthesis of highly fluorescent nitrogen-doped graphene quantum dots for sensitive, label- free detection of Fe (III) in aqueous media. Biosens Bioelectron 58, 219-225.
[22] Kleine-Vehn J, Ding ZJ, Jones AR, Tasaka M, Morita MT, Friml J (2010). Gravity-induced PIN transcytosis for polarization of auxin fluxes in gravity-sensing root cells. Proc Natl Acad Sci USA 107, 22344-22349.
[23] Kong Z, Hu W, Jiao FF, Zhang PZ, Shen JW, Cui B, Wang HB, Liang LJ (2020). Theoretical evaluation of DNA genotoxicity of graphene quantum dots: a combination of density functional theory and molecular dynamics simulations. J Phys Chem B 124, 9335-9342.
[24] Ku TT, Hao F, Yang XX, Rao ZY, Liu QS, Sang N, Faiola F, Zhou QF, Jiang GB (2021). Graphene quantum dots disrupt embryonic stem cell differentiation by interfering with the methylation level of Sox2. Environ Sci Technol 55, 3144-3155.
[25] Leitz G, Kang BH, Schoenwaelder ME, Staehelin LA (2009). Statolith sedimentation kinetics and force transduction to the cortical endoplasmic reticulum in gravity- sensing Arabidopsis columella cells. Plant Cell 21, 843-860.
[26] Li XL, Zhou ZH, Lu DJ, Dong XW, Xu MH, Wei LM, Zhang YF (2014). The effect of pristine carbon-based nanomaterial on the growth of green gram sprouts and pH of water. Nanoscale Res Lett 9, 583.
[27] Li Y, Yuan W, Li LC, Miao R, Dai H, Zhang JH, Xu WF (2020). Light-dark modulates root hydrotropism associated with gravitropism by involving amyloplast response in Arabidopsis. Cell Rep 32, 108198.
[28] Li Y, Zhao Y, Cheng HH, Hu Y, Shi GQ, Dai LM, Qu LT (2012). Nitrogen-doped graphene quantum dots with oxygen-rich functional groups. J Am Chem Soc 134, 15-18.
[29] Lin YH, Zhuang SX, Wang YL, Lin S, Hong ZW, Liu Y, Xu L, Li FP, Xu BH, Chen MH, He SW, Liao BQ, Fu XP, Jiang ZQ, Wang HL (2019). The effects of graphene quantum dots on the maturation of mouse oocytes and development of offspring. J Cell Physiol 234, 13820-13831.
[30] Morita MT, Tasaka M (2004). Gravity sensing and signaling. Curr Opin Plant Biol 7, 712-718.
[31] Nan WB, Wang XM, Yang L, Hu YF, Wei YT, Liang XL, Mao LN, Bi YR (2014). Cyclic GMP is involved in auxin signaling during Arabidopsis root growth and development. J Exp Bot 65, 1571-1583.
[32] Ottenschl?ger I, Wolff P, Wolverton C, Bhalerao RP, Sandberg G, Ishikawa H, Evans M, Palme K (2003). Gravity-regulated differential auxin transport from columella to lateral root cap cells. Proc Natl Acad Sci USA 100, 2987-2991.
[33] Su SH, Gibbs NM, Jancewicz AL, Masson PH (2017). Molecular mechanisms of root gravitropism. Curr Biol 27, R964-R972.
[34] Sun HF, Wang M, Wang J, Wang WP (2022). Surface charge affects foliar uptake, transport and physiological effects of functionalized graphene quantum dots in plants. Sci Total Environ 812, 151506.
[35] Swarup R, Kramer EM, Perry P, Knox K, Leyser HMO, Haseloff J, Beemster GTS, Bhalerao R, Bennett MJ (2005). Root gravitropism requires lateral root cap and epidermal cells for transport and response to a mobile auxin signal. Nat Cell Biol 7, 1057-1065.
[36] Tsugeki R, Fedoroff NV (1999). Genetic ablation of root cap cells in Arabidopsis. Proc Natl Acad Sci USA 96, 12941-12946.
[37] Ulmasov T, Murfett J, Hagen G, Guilfoyle TJ (1997). Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Plant Cell 9, 1963-1971.
[38] Wang D, Zhu L, Chen JF, Dai LM (2015a). Can graphene quantum dots cause DNA damage in cells? Nanoscale 7, 9894-9901.
[39] Wang ZG, Zhou R, Jiang D, Song JE, Xu Q, Si J, Chen YP, Zhou X, Gan L, Li JZ, Zhang H, Liu B (2015b). Toxicity of graphene quantum dots in zebrafish embryo. Biomed Environ Sci 28, 341-351.
[40] Wen J, Xu YQ, Li HJ, Lu AP, Sun SG (2015). Recent applications of carbon nanomaterials in fluorescence biosensing and bioimaging. Chem Commun 51, 11346-11358.
[41] Wis?niewska J, Xu J, Seifertová D, Brewer PB, Ru?z?ic?ka K, Blilou I, Rouquié D, Benková E, Scheres B, Friml J (2006). Polar PIN localization directs auxin flow in plants. Science 312, 883.
[42] Zheng XT, Ananthanarayanan A, Luo KQ, Chen P (2015). Glowing graphene quantum dots and carbon dots: properties, syntheses, and biological applications. Small 11, 1620-1636.
[43] Zhu JK (2016). Abiotic stress signaling and responses in plants. Cell 167, 313-324.
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