Applications of Optical Clearing Technique in Multi-scale Imaging
†These authors contributed equally to this paper
Received date: 2021-10-11
Accepted date: 2022-01-13
Online published: 2022-01-13
The optical clearing techniques are common methods that allow biological tissues or organs to be deeply transparent and imaged under an optical microscope by processing with chemical reagents. Driven by a variety of optical microscopy technologies, the entire tissue can be imaged and reconstructed three-dimensionally (3D) using the clearing technique to deeply analyze the internal spatial characteristics and formation mechanism of biological tissues. In recent years, several optical clearing techniques and multi-scale imaging techniques have been developed and fruitful researches have been reported. This article discusses basic principles and some new techniques of optical clearing, with particular focus on new imaging methods based on optical clearing technique and their applications in plant imaging and cell biology. This review will provide theoretical basis and technical support for the subsequent research on clearing, imaging and 3D reconstruction and function of whole plants, tissues or organs.
Lingyu Ma, Xiaohong Qi, Zijian Hu, Weiwei Shen, Guangchao Wang, Bolin Zhang, Xi Zhang, Jinxing Lin . Applications of Optical Clearing Technique in Multi-scale Imaging[J]. Chinese Bulletin of Botany, 2022 , 57(1) : 98 -110 . DOI: 10.11983/CBB21175
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