Molecular Identification of Tomato Spotted Wilt Virus on Tomato in Yinchuan
- 1School of Agriculture, Ningxia University, Yinchuan 750021, China
2Ningxia Modern Facility Horticulture Engineering Technology Research Center, Yinchuan 750021, China
3Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, Yinchuan 750021, China
4Ningxia Facility Horticulture (Ningxia University) Technology Innovation Center, Yinchuan 750021, China
5College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
Received date: 2021-07-09
Accepted date: 2021-11-20
Online published: 2021-11-17
Abstract
In order to determine whether Yinchuan tomato (Lycopersicon esculentum) was damaged by tomato spotted wilt virus (TSWV), 14 disease leaf samples of suspected infected TSWV collected from Yinchuan tomato were identified by the national standard TSWV RT-PCR technique. The cloned N gene sequences were analyzed by multi sequence alignment and phylogenetic tree analysis. Eight of the 14 disease leaf samples amplified the TSWV N gene sequence with a length of 394 bp, and the 8 sequences were completely consistent. The TSWV sequence was relatively close to those from Yunnan tomato, Chinese lettuce (Lactuca sativa), Chinese Iris (Iris tectorum) and Chongqing pepper (Capsicum annuum), while relatively distant to those from Shandong, Heilongjiang, Beijing and the abroad. Detection of TSWV in the 8 PCR positive samples by use of Western bolt showed that TSWV infection existed in these samples. This study proved that there was TSWV infection in Yinchuan tomato. Therefore, it is necessary to speed up the breeding of TSWV resistant tomato varieties.
Key words： Yinchuan; tomato spotted wilt virus; RT-PCR; Western blot
Cite this article
Xiaomin Wang, Honglei Li, Lin Wang, Pengze Zhou, Shengyi Bai, Guohua Li, Fushun Zheng, Xiaorong Tao, Guoxin Cheng, Yanming Gao, Jianshe Li . Molecular Identification of Tomato Spotted Wilt Virus on Tomato in Yinchuan[J]. Chinese Bulletin of Botany, 2021 , 56(6) : 715 -721 . DOI: 10.11983/CBB21113
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