Clustering Analysis of Volatile Components from the Tea Plants Infested by Tea Aphid (Toxoptera aurantii)
† These authors contributed equally to this paper
Received date: 2021-02-07
Accepted date: 2021-04-19
Online published: 2021-04-21
Shuchazao tea (Camellia sinensis cv. ‘shuchazao’) is a newly cultivated tea species in southwest Anhui, China. To investigate the volatile release profiles of tea aphid (Toxoptera aurantii) infested tea shoots, gas chromatography-mass spectrometry (GC-MS) analysis was used to compare the volatile compositions and relative contents in healthy and infested tea shoots. 16 volatile organic compounds (VOCs) were detected in healthy tea shoots with fewer relative contents, whereas more volatiles (24 VOCs) were released with increasing relative contents in tea plants induced by tea aphid feeding. Unsupervised clustering analysis based on the significantly different volatiles indicated that the volatile profiles in healthy and tea aphid infested tea shoots apparently showed clustering characteristics. A model was created by supervised partial least square discrimination analysis (PLS-DA) for volatiles with statistical significance. By experimental verification, the model could clearly discriminate healthy tea shoots from aphid infested ones (R2X=0.903, R2Y=0.875). By calculating the variable importance for the projection (VIP), seven important volatiles (α-pinene, longifolene-(V4), benaldehyde, E-5,9-undecadien-2-one,6,10-dimethyl-, 3-hexenal, camphor and decanal) were identified which collectively contribute to discrimination between healthy and infested tea shoots. The current work has preliminarily demonstrated the changing patterns in tea plant volatiles after tea aphid infestation to provide novel theoretical guidance for tea aphid management.
Key words： tea plant volatiles; GC-MS; PLS-DA; tea aphid; clustering analysis
Tingzhe Sun, Zehua Qi, Kexin Liang, Qin Li, Yuchun Rao, Dan Mu . Clustering Analysis of Volatile Components from the Tea Plants Infested by Tea Aphid (Toxoptera aurantii)[J]. Chinese Bulletin of Botany, 2021 , 56(4) : 422 -432 . DOI: 10.11983/CBB21031
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