A Rapid, Non-destructive and Continuous Sampling Technique and DNA Extraction for Soybean Seed
- 1Northeast Institute of Geography and Agroecology, the Innovative Academy of Seed Design, Chinese Academy of Sciences, Harbin 150081, China
2University of Chinese Academy of Sciences, Beijing 100049, China
Received date: 2020-05-26
Accepted date: 2020-10-05
Online published: 2020-10-11
Abstract
Establishment of a simple, quick, non-destructive, and continuous sampling procedure, can save planting cost and accelerate gene functional analysis and breeding procedures. In this study, we created a rapid, non-destructive, and continuous sampling technique using micro electric driller and air pump. We also optimized the high throughput DNA extraction method for the 384 deep-well plate and genotyping method. Furthermore, this technique could be applied in rice, maize and other crops for seed sampling and high throughput genotyping.
Key words： soybean (Glycine max); seed; drilling; continuing sampling; 384 deep-well plate; genotyping
Cite this article
Zhengjun Xia, Yuzhuo Li, Jinlong Zhu, Hongyan Wu, Kun Xu, Hong Zhai . A Rapid, Non-destructive and Continuous Sampling Technique and DNA Extraction for Soybean Seed[J]. Chinese Bulletin of Botany, 2021 , 56(1) : 56 -61 . DOI: 10.11983/CBB20095
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