Received date: 2020-05-08
Accepted date: 2020-08-13
Online published: 2020-08-26
Chromatin immunoprecipitation (ChIP) is a teschnique used to investigate protein-DNA interaction. Briefly, protein and associated DNA are crosslinked, which is optional. Then the DNA-protein complexes are fragmented. By using an appropriate antibody, cross-linked DNA fragments associated with the protein of interest are selectively immunoprecipitated. ChIP is commonly used to identify binding regions of DNA-binding proteins, such as transcription factors. ChIP is also used to analyze histone modification profiles in combination with antibodies against specific histone modifications. Here we describe protocols and related tips for cross-linked ChIP and ultra-low-input micrococcal nuclease-based native ChIP (ULI-NChIP), which can be applied to rare cell populations at the 103 cell level.
Hongli Wang,Yuling Jiao . Protocols for Chromatin Immunoprecipitation[J]. Chinese Bulletin of Botany, 2020 , 55(4) : 475 -480 . DOI: 10.11983/CBB20076
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