TECHNIQUES AND METHODS

Luciferase Complementation Assay for Detecting Protein Interactions

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  • Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Received date: 2019-11-24

  Accepted date: 2019-12-02

  Online published: 2019-12-04

Abstract

Protein-Protein interactions play important roles in various eukaryotic biological processes. Compared to other techniques measuring protein-protein interactions in plants, the Luciferase Complementation Assay (LCA), based on Agrobacterium-mediated transient expression in Nicotiana benthamiana, is a simple, sensitive, reliable, highly quantitative and low background method that can be easily scaled up for high-throughput interactome studies. Here, we describe a protocol that includes two alternative data collection methods to qualitative and quantitative analyse luminescence or luminous intensity to detect protein-protein interactions in plant cells.

Cite this article

Yan Zhao,Jianmin Zhou . Luciferase Complementation Assay for Detecting Protein Interactions[J]. Chinese Bulletin of Botany, 2020 , 55(1) : 69 -75 . DOI: 10.11983/CBB19229

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