Expression Patterns and Bioinformatic Analyses of Salt Stress Responsive Gene IbMYB3 in Ipomoea batatas
Received date: 2019-05-22
Accepted date: 2019-11-27
Online published: 2019-12-04
MYB transcription factors have multiple biological functions and play important roles in mediating plant responses to biotic and abiotic stresses. In this paper, two MYB genes, named IbMYB3 and IbMYB4, which were significantly induced by salt stress, were screened from RNA-seq data of salt-stressed sweetpotato (Ipomoea batatas) plantlets. Gene expression analysis showed that the expression of IbMYB3 was significantly up-regulated by various abiotic stresses and plant growth substances treatments, suggesting that IbMYB3 might be involved in the abiotic stress responses of sweetpotato. Further bioinformatic analysis showed that the open reading frame of IbMYB3 is 1 059 bp in length, encoding 353 amino acids, with a predicted molecular weight of 39.41 kDa and the theoretical isoelectric point (PI) of 5.26, which is an acidic negatively charged hydrophilic protein. Subcellular localization showed that the IbMYB3 protein localizes to the nucleus, and has strong transcriptional activation activity. Taken together, these results demonstrated that the IbMYB3 transcription factor might play an important role in regulating the abiotic stress responses of sweetpotato. This study thus lays the foundation for further illustration of IbMYB3 function.
Ge Li,Xiaoqing Meng,Zongyun Li,Mingku Zhu . Expression Patterns and Bioinformatic Analyses of Salt Stress Responsive Gene IbMYB3 in Ipomoea batatas[J]. Chinese Bulletin of Botany, 2020 , 55(1) : 38 -48 . DOI: 10.11983/CBB19094
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