TECHNIQUES AND METHODS

Protocols for Analyzing Rice Meiotic Chromosomes

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  • 1 Agriculture College, Yangzhou University, Yangzhou 225009, China
    2 Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Received date: 2019-05-12

  Accepted date: 2019-06-14

  Online published: 2019-06-17

Abstract

The development of techniques to analyze pachytene chromosomes has greatly overcome most of the difficulties in cytological studies of rice chromosomes caused by their small size. Visualization of meiotic chromosomes has now become routine in cytogenetic studies of this species. This chapter provides protocols on basic meiotic chromosome preparation, FISH analysis and immunocytology in rice.

Cite this article

Xinjie Cheng, Hengxiu Yu, Zhukuan Cheng . Protocols for Analyzing Rice Meiotic Chromosomes[J]. Chinese Bulletin of Botany, 2019 , 54(4) : 503 -508 . DOI: 10.11983/CBB19087

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