By examining 40 cut chrysanthemum varieties, we obtained 6 qualitative classification characters and 14 leaf structure parameters, including ratio of leaf length to width, extent of acumination of leaf tip, ratio of lobe length to width, and notch acuity of lobe. We selected 18 varieties with similar leaf appearance after clustering by the 6 qualitative classification traits. The 18 varieties were differentiated numerically by multiple discriminant analysis. Varieties with similar leaf appearance were distinguished, and the mean identifiable fitness of the 18 cultivars was 88.28% on multiple discriminant analysis. With these leaf measurement data, we can effectively differentiate different cultivars of cut chrysanthemum.