Spore Germination and Callus Induction of Atrichum undulatum

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  • School of Life Sciences, Capital Normal University, Beijing 100048, China

Received date: 2012-09-12

  Revised date: 2012-12-13

  Online published: 2013-12-03

Abstract

We studied the spore germination and protonema development of Atrichum undulatum collected from Wuling Mountain, Hebei Province (1 500 m a.s.l.). The dormancy stage is absent during spore development. Spores in incubation culture take 3 days to germinate. Spores germinate exogenously by forming a protonema in 2 growth phases: chloronema and caulonema. The best growth conditions were MS medium with 2% glucose at 25°C/20°C, 14 h light/10 h dark, 36 μmol·m–2·s–1. The optimal medium for callus induction is MS medium supplemented with 2% glucose and 1.0 mg?L–1 6-BA to obtain high-quality cormus for large-scale cultivation.

Cite this article

Xiaofeng Wu, Ruoyang Hu, Xuedong Li . Spore Germination and Callus Induction of Atrichum undulatum[J]. Chinese Bulletin of Botany, 2013 , 48(6) : 651 -657 . DOI: 10.3724/SP.J.1259.2013.00651

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