Analysis of Differentially Expressed Genes in Suaeda liaotungensis NAC4 (SlNAC4)-transgenic Arabidopsis
Received date: 2016-01-08
Revised date: 2016-06-15
Online published: 2016-12-02
Microarray assay was used for gene expression profiling of Suaeda liaotungensis NAC4 (SlNAC4)-transgenic Arabidopsis and the wild type. SlNAC4-transgenic Arabidopsis showed 3 094 differentially expressed genes as compared with the wild type. Gene ontology analysis revealed 195 differentially expressed genes related to abiotic stress and 47 related to growth and development, including MYB and WRKY transcription factor genes. KEGG analysis revealed the differentially expressed genes involved in 90 signaling pathways. The main signaling pathways were plant hormone signal transduction and brassinosteroid synthesis. The differential expression of selected genes was validated by qRT-PCR. SlNAC4 regulated the expression of downstream genes directly and indirectly, functioning in plant growth and development and abiotic stress tolerance.
Qianqian Li, Yang Jiao, Jingyang Yu, Qiuli Li . Analysis of Differentially Expressed Genes in Suaeda liaotungensis NAC4 (SlNAC4)-transgenic Arabidopsis[J]. Chinese Bulletin of Botany, 2016 , 51(6) : 764 -773 . DOI: 10.11983/CBB16004
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