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Scanning for Pi Loci and Rebuilding an Improved Genome of Elite Rice Variety Kongyu 131

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  • Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101

# Co-first authors

Received date: 2016-05-11

  Accepted date: 2016-06-15

  Online published: 2017-01-23

Abstract

The rice variety Kongyu 131 (Oryza sativa ssp. japonica) is the most widely grown elite cultivar in Heilongjiang province because of its high quality, early maturity, high yield and cold resistance. However, because it has been cultivated in the same areas for many years, it is now highly susceptible to rice blast. By re-sequencing and scanning the whole genome of Kongyu 131, we found that it lacks more than 12 cloned blast resistance genes, including Pi2, Pi9, Pi36, Pi5-1, Pb1, Pid3, Pi25, Pikh, Pi1, Pik-m, Pik-p and Pi56t. To improve the blast resistance, we successfully introgressed Pb1 (Panicle blast 1) into Kongyu131. Without changing the other agronomic characters, we used 5 single nucleotide polymorphism markers to control the length of the introduced target fragment derived from the donor cultivar MP. Inoculating test results indicated that the improved line, containing a 700 kb target fragment and sharing 99.38% genetic background with Kongyu 131, showed the same blast resistance as MP.

Cite this article

Xiaohui Zhang, Xiaomin Feng, Shaoyang Lin . Scanning for Pi Loci and Rebuilding an Improved Genome of Elite Rice Variety Kongyu 131[J]. Chinese Bulletin of Botany, 2017 , 52(1) : 30 -42 . DOI: 10.11983/CBB16107

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