Effect of Paclobutrazol on Growth and Production of Esculentoside A in Hairy Roots of Phytolacca americana
# Co-first authors
Received date: 2015-12-10
Accepted date: 2016-06-13
Online published: 2016-12-02
To explore the possibility of the use of the plant growth regulator paclobutrazol (PP333) to regulate growth and secondary metabolism of hairy roots, we examined the effect of PP333 on the growth and production of esculentoside A in Phytolacca americana hairy roots. Compared to the control, with increasing concentration of PP333 in the medium, the lateral roots of hairy roots became short and thick and the root tips and surface of lateral roots turned light red and the root surface became redder. The addition of 0.5-5.0 mg∙L-1 PP333 in the medium promoted the production of esculentoside A in hairy roots. The highest content of esculentoside A, 6.22 mg∙g-1 dry weight, about 1.94 times that of the control, was obtained with 1.0 mg∙L-1 PP333. The enhanced production of esculentoside A by PP333 may be related to its regulation of phenylalanine ammonia lyase activities of P. americana hairy roots.
Xizi Shi, Yapeng Guo, Heping Shi . Effect of Paclobutrazol on Growth and Production of Esculentoside A in Hairy Roots of Phytolacca americana[J]. Chinese Bulletin of Botany, 2016 , 51(6) : 801 -806 . DOI: 10.11983/CBB15220
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