# Co-first authors
Received date: 2016-01-13
Accepted date: 2016-05-12
Online published: 2016-09-27
The mature seeds of Scirpus × mariqueter were used as explants. Through germ-free germination, induction of multiple shoots, multiplication, rooting and transplantion, an aseptic-frequency regeneration system was established. The optimal medium for inducing adventitious plants and multiplication was MS+2.0 mg·L-1 6-BA+0.002 mg·L-1 TDZ+0.2 mg·L-1 IBA; the optimal medium for culturing strong plants with multiple shoots was 1/2MS+0.05 mg·L-1 6-BA+0.01 mg·L-1 IBA; the optimal medium for rooting was 1/2MS+0.2 mg·L-1 IBA; and the optimal culture temperature was 30°C. Regenerated seedlings were transplanted into the mixture of 3 parts perlite, turf and vermiculite, volume ratio 1:1:1, and the survival rate was >85%. A large-volume plastic container for rooting culture can decrease the cost of production and increase production efficiency.
Qun Zhang, Xiuli Lü, Xiaoli He, Yi Zhu, Xinhong Cui . A Rapid Propagation System for Scirpus × mariqueter[J]. Chinese Bulletin of Botany, 2016 , 51(5) : 684 -690 . DOI: 10.11983/CBB16008
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