# Co-first authors
Received date: 2016-01-15
Accepted date: 2016-04-29
Online published: 2017-01-23
Dwarf rice (Oryza sativa) is the most important agronomic trait closely related to photosynthetic efficiency and yield. Exploring more dwarf resources can deepen our understanding of the genetic mechanisms of plant height and provide new germplasm for cultivating new varieties. A wide-leaf, wide-stem, small-kernel and dwarf mutant, wld1, was identified from the rice T-DNA insertion mutant library. We used map-based cloning to locate the gene on rice chromosome 5 between the markers InDel37 and InDel48, with 91 kb physical distance. The mutant wld1 resulted from deletion of the base T in LOC_Os05g32270, which causes a reading error and early termination of the peptide chain encoding. We used microscopy to observe the top-second internal structure of vegetative organs of the mutant and the wild type in paraffin sections. In the wld1, cells were significantly increased in size in cross-sections but reduced in size in longitudinal sections. The expression of LOC_Os05g32270 was completely inactivated. The gene is an allele of the reported rice gene OsSMOS1 (SMALL ORGAN SIZE1). The agronomic properties of wld1 are good (lodging) and it can be directly applied to practice. In this study, we defined the inheritance and phenotype characteristics of this mutant, which lays the foundation for further analysis of the signaling pathway.
Wang Wenle, Feng Dan, Wu Jinxia, Zhang Zhiguo, Lu Tiegang . Gene Mapping of a Dwarf Gene WLD1 in Rice[J]. Chinese Bulletin of Botany, 2017 , 52(1) : 54 -60 . DOI: 10.11983/CBB16010
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