Bi and Cl genes, encoding anthocyanin synthesis of transcription factor, were used as visual selection reporter genes, and the glyphosate-resistance gene EPSPS as a selective marker, to generate a visualizing track vector pBAC9017. We used this vector to transform Cry1Ab/c driven by an enhanced CaMV 35S (E35S) promotor, into immature embryos and embryonic callus of maize inbred line 501 by microprojectile bombardment. We obtained 147 transformed, and among 106 plants with harvested T0 ears, 16 ears were decorated with mosaic purple seeds. PCR analysis showed that the Cry1Ab/c gene was integrated into the genome of maize. Extracts of seed protein were tested by BT-Cry1Ab/1Ac immune-strip and ELISA, showing that Bt-Cry1Ab/c gene was expressed in transgenic lines.
Chaojie Ba, Jing Xue, Xuqing Chen, Fengping Yang, Liquan Zhang, Xianglong Li, Xiaodong Zhang
. Rapid Screening of Cry1Ab/c Transgenic Maize Using an Anthocyanin Visualizing Track System[J]. Chinese Bulletin of Botany, 2013
, 48(1)
: 59
-64
.
DOI: 10.3724/SP.J.1259.2013.00059
陈健, 孙爱东, 高雪娟, 陶晓赟, 王姗姗 (2011). 蓝莓花青素的提取及抗氧化性的研究. 北京林业大学学报 33, 126－129.
宫硖, 薛静, 张晓东 (2011). 植物花青素合成途径中的调控基因研究进展. 生物技术进展 1, 381－390.
孙宝刚, 郑剑英, 戴福全, 袁世权, 徐海艳(2010). 玉米螟生物防治技术. 吉林农业. pp. 84.
王艳辉, 陈旭清, 薛静, 张立全, 杨凤萍, 李向龙, 张晓东(2010). Cry1Ab/1Ac基因在转基因玉米中的表达研究. 分子植物育种 8, 1－6.
吴才君, 范淑英 (2004). 植物转基因沉默. 江西农业大学学报 26, 154－158.
Bohorova N, Zhang W, Julstrum P, Mclean S, Luna B, Brito RM, Diaz L, Ramos ME, Estanol P, Pacheco M, Salgado M, Hoisington D(1999). Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos. Theor Appl Genet 99, 437－444.
Crickmore N, Zeigler DR, Feitelson J, Schnepf E, Van RJ, Lereclus D, Baum J, Dean DH(1998). Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol Mol Biol Rev 62, 807－813.
Sauka DH, Amadio AF, Zandomeni RO, Benintende GB(2007). Strategy for amplification and sequencing of insecticidal cry1A genes from Bacillus thuringiensis, Antonie van Leeuwenhoek 91, 423－430.
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