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Chinese Bulletin of Botany >

2013 , Vol. 48 >Issue 1: 79 - 86

DOI: https://doi.org/10.3724/SP.J.1259.2013.00079

In Vitro Culture of Pumpkin Unfertilized Ovules and Plant Regeneration

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  • College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2012-07-27

  Revised date: 2012-09-30

  Online published: 2012-11-01

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Abstract

We used unfertilized ovules of the pumpkin cultivar ‘Experiment 1’ as explants to examine the effect of combinations and concentrations of growth regulators, different developmental periods, high-temperature pretreatment, and AgNO3 concentrations on embryoid induction. The combination of 2,4-D, NAA, and 6-BA favoured embryoid induction; the medium with the best effect was Murishige and Skoog (MS)+1.0 mg·L–12,4-D+0.25 mg·L–1NAA+0.5 mg·L–1 6-BA, for 31.1% embryoid induction. The flowering ovules for the day showed the highest frequency of embryoid induction (26.7%) and the lowest frequency of callus formation (<5%). The explants treated with darkness and high temperature (35°C) for 5 days showed the best embryoid induction, 32.2%. However, the addition of AgNO3 remarkably inhibited embryoid induction. Transfer of embryoids to plant-regeneration medium led to the highest regeneration frequency, 64.3%. Plant regeneration expressed a typical pathway as for embryogenesis. Cytologic observations revealed that embryoids probably derived from cells close to the micropyle (i.e., ovums or synergids).

Key words： embryoid; in vitro culture; plant regeneration; pumpkin; unfertilized ovules

Cite this article

Shouru Sun, Peng Zhang, Jianbin Hu, Liping Sun, Man Zhang, Zhiqiang Sun . In Vitro Culture of Pumpkin Unfertilized Ovules and Plant Regeneration[J]. Chinese Bulletin of Botany, 2013 , 48(1) : 79 -86 . DOI: 10.3724/SP.J.1259.2013.00079

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