Dynamic Changes in Activity and Distribution of Proteasome During Pollen Development of Pinus bungeana

Expand
  • 1College of Life Science, Capital Normal University, Beijing 100048, China

    2Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received date: 2011-08-23

  Revised date: 2011-11-30

  Online published: 2012-03-16

Abstract

Although the proteasome pathway has long been considered one of the most important proteolytic systems in pollen development, the dynamic changes of proteasome activity and distribution during pollen development are largely unknown. We performed fluorogenic kinetic assays using fluorogenic substrate sLLVY-AMC to measure proteasome activity in Pinus bungeana pollen grains. Our experiment results revealed that proteasome activity increased from the uninucleate to 3-cell stage, but slightly decreased in mature pollen grains. Immunofluorescence labelling detected proteasome signals in the nuclei of pollen grains at all stages as well as in cytosol. Unequal distribution of proteasomes was observed in the two degradating prothallial cells. Our data indicate a link between pollen development and dynamic changes in activity and distribution of proteasome, and proteasome might take a part in the degradation of prothallial cells.

Cite this article

Liping Jiang, Xiaoling Dong, Xue Li, Yuan Gao, Xianyong Sheng . Dynamic Changes in Activity and Distribution of Proteasome During Pollen Development of Pinus bungeana[J]. Chinese Bulletin of Botany, 2012 , 47(2) : 141 -148 . DOI: 10.3724/SP.J.1259.2012.00141

References

[1]林金星,胡玉熹.裸子植物结构图集200
[2]盛仙永, 胡正海, 林金星.泛素/蛋白酶体途径及其在高等植物有性生殖中的作用[J].西北植物学报,2004,24:1527-1536
[3]Alche JD, Butowt R, Castro AJ, Rodriguez-Garcia MI.Ubiquitin and ubiquiin-conjugated protein in the olive (Olea europaea L) pollen[J]..Sex Plant Reprod,2000,12:285-291
[4]Book AJ, Smalle J, Lee K, Yang PZ, Walker JM, Casper S, Holmes JH, Russo LA, Zachri W, Buzzinotti ZW, Jenik PD, Vierstra RD.The RPN5 subunit of the 26s proteasome is essential for gametogenesis, sporophyte development, and complex assembly in Arabidopsis[J].Plant Cell,2009,21:460-478
[5]Brukhin V, Gheyselinck J, Genschik P, Grossniklaus U.The RPN1 subunit of the 26S proteasome in Arabidopsis is essential for embryogenesis[J].Plant Cell,2005,17:2723-2737
[6]Ciechanover A.Intracellular protein degradation: from a vague idea thru the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting[J].Cell Death Differ,2005,12:1178-1190
[7]Doelling JH, Phillips AR, Soyler-Ogretim G, Wise J, Chandler J, Callis J, Otegui MS, Vierstra RD.The ubiquitin-specific protease subfamily UBP3/UBP4 is essential for pollen development and transmission in Arabidopsis[J].Plant Physiol,2007,145:801-813
[8]Doelling JH, Yan N, Kurepa J, Walker J, Vierstra RD.The ubiquitin-specific protease UBP14 is essential for early embryo development in Arabidopsis thaliana[J].Plant J,2001,27:393-405
[9]Gallois J-L, Guyon-Debast A, Lecureuil A, Vezon D, Carpentier V, Bonhomme S, Guerchea P.The Arabidopsis proteasome rpt5 subunits are essential for gametophyte development and show accession-dependent redundancy[J].Plant Cell,2009,21:442-459
[10]Hershko A.The ubiquitin system for protein degradation and some of its roles in the control of the cell division cycle[J].Cell Death Differ,2005,12:1191-1197
[11]Kim M, Ahn JW, Jin UH, Choi D, Paek KH, Pai HS.Activation of the programmed cell death pathway by inhibition of proteasome function in plants[J].J Biol Chem,2003,278:19406-19415
[12]Li YQ, Southworth D, Linskens HF, Mulcahy DL, Cresti M.Localization of ubiquitin in anthers and pistils of Nicotiana[J].Sex Plant Reprod,1995,8:123-128
[13]Lu SY, Li YF, Chen ZK, Lin JX.Pollen development in Picea asperata MAST[J].Flora,2003,198:112-117
[14]McCormick S.Control of male gametophyte development[J].Plant Cell,2004,16:142-
[15]Palmer A, Mason GG, Paramio JM, Knecht E, Rivett AJ.Changes in proteasome localization during the cell cycle[J].Eur J Cell Biol,1994,64:163-175
[16]Peters JM, Franke WW, Kleinschmidt JA.Distinct 19S and 20S subcomplexes of the 26S proteasome and their distribution in the nucleus and the cytoplasma[J].J Biol Chem,1994,269:7709-7718
[17]Reits EA, Benham AM, Plougastel B, Neefjes J, Trowsdale J.Dynamics of proteasome distribution in living cells[J].EMBO J,1997,16:6087-6094
[18]Rivett AJ, Palmer A, Knecht E.Electron microscopic localization of the multicatalytic proteinase complex in rat liver and in cultured cells[J].J Histochem Cytochem,1992,40:1165-1172
[19]Scoccianti V, Speranzab A, Crinellic R, Calzonib GL, Biasid R, Altamura MM, Bagnib N.Development-related changes of protein ubiquitination in pollen from male and female kiwifruit (Actinidia deliciosa)[J].Physiol Plantarum,1999,107:128-135
[20]Scott RJ, Spielman M, Dickinson HG.Stamen structure and function[J].Plant Cell,2004,16:46-
[21]Sheng XY, Dong XL, Zhang SS, Jiang LP, Tan LL, Li X.Unequal distribution of ubiquitinated proteins during Pinus bungeana pollen development[J].Trees,2011,25:407-414
[22]Sheng XY, Dong XL, Zhang SS, Jiang LP, Zhu J, Wang L.Mitochondrial dynamics and its responds to proteasome defection during Picea wilsonii pollen tube development[J].Cell Biochem Funct,2010,28:420-425
[23]Sheng XY, Hu ZH, Lu HF, Wang XH, Baluska F, Samaj J, Lin JX.Roles of the ubiquitin/proteasome pathway in pollen tube growth with emphasis on MG132-induced alterations in ultrastructure, cytoskeleton, and cell wall components[J].Plant Physiol,2006,141:1578-1590
[24]Speranza A, Scoccianti V, Crinelli R, Calzoni GL, Magnani M.Inhibition of proteasome activity strongly affects kiwifruit pollen germinationInvolvement of the ubiquitin/proteasome pathway as a major regulator[J]..Plant Physiol,2001,126:1150-1161
[25]Wojcik C, DeMartino GN.Intracellular localization of proteasomes[J].Int J Biochem Cell Biol,2003,35:579-589
Outlines

/