In this research, to establish a tissue culture and rapid propagation system of Ardisia gigantifolia, tender stem segments was selected as explants, and axillary buds were used to study proliferation. Culture medium of MS+1.0 mg·L ^-16-BA+0.2 mg·L ^-1 NAA and MS+0.5 mg·L ^-1 ZT had a significant effect on axillary bud induction and subculture of previous generations; the induction rates were 89.3% and 85.7%, respectively. The best culture medium for the multiplication was MS+0.5 mg·L ^-1 6-BA+0.1 mg·L ^-1 ZT+0.1 mg·L ^-1 NAA, and the propagation coefficient was 4.3. The optimal medium for rooting was 1/2MS+1.5 mg·L ^-1 IAA+1.0 mg·L ^-1NAA; the rooting rate reached 92.3%. The roots developed well and plantlets grew vigorously. Plantlets were transplanted to garden soil:peat soil:perlite=3:1:1 (v/v/v), and 82% rooted plants survived. The study established a fast propagation system for A. gigantifolia that could be applied to factory production.