Bioinformatics Analysis of tRNA-derived Fragments in Rice Male Gametophyte Development
† These authors contributed equally to this paper
Received date: 2017-09-06
Accepted date: 2017-11-16
Online published: 2018-11-29
tRNA derived fragments (termed tRFs) are functional RNAs produced by non-random cleavage of tRNAs. The knowledge of tRNAs’ production and function is being studied but is unknown in the rice male gametophyte. Using high-throughput sequencing with 3 stages in the rice male gametophyte development, we found tRFs with a large range of length. Next, we determined four sequence-specific and one non-sequence-specific ribonuclease cleaving sites. We also summarized the distribution profile of tRFs in the three stages. Finally, a NCBI blast search was carried out to predict the tRF target gene, revealing that the major class of targets was transposable elements. This results provid new clues for rice male gametophyte research.
Key words： rice; male gametophyte; pollen; tRFs; bioinformatics
Liu Wei, Tong Yong’ao, Bai Jie . Bioinformatics Analysis of tRNA-derived Fragments in Rice Male Gametophyte Development[J]. Chinese Bulletin of Botany, 2018 , 53(5) : 625 -633 . DOI: 10.11983/CBB17169
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