Rooting Induced on in vitro Leaves and Fresh Cuttings of Populus euphratica

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  • 1College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
    2Beijing Yanqing District First Vocational School, Beijing 102100, China

# Co-first authors

Received date: 2016-02-04

  Accepted date: 2016-04-26

  Online published: 2017-04-05

Abstract

Populus euphratica is the unique natural distribution arbor tree growing in the dry desert environment, with root suckering, but cuttings of roots are difficult to grow. This study explored the new ways of rooting induced by Agrobacterium rhizogenes. The strain ATCC11325 can induce hairy roots with no-tropism on in vitro leaf sections. The leaves were cut, scarred, co-cultivated, soaked for 10 min in the microbial agent at OD600=0.4 with AS 100 mg·L-1 added and cultured on tissue culture media for 20 days. Hairy rooting occurred on fresh cuttings soaked with the A. rhizogenes strain solution. The rooting and survival rate reached 60%, and aboveground parts developed more branches and showed vigorous growth. The A. rhizogenes strain promoted rooting of poplar tree fresh cuttings.

Cite this article

Dapeng Wang, Jiaze Tang, Mingcheng Shao, Wenbiao Zhang, Huafang Wang . Rooting Induced on in vitro Leaves and Fresh Cuttings of Populus euphratica[J]. Chinese Bulletin of Botany, 2017 , 52(2) : 210 -217 . DOI: 10.11983/CBB16024

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