Chinese Bulletin of Botany >

2018 , Vol. 53 >Issue 1: 42 - 50

DOI: https://doi.org/10.11983/CBB17058

EXPERIMENTAL COMMUNICATIONS

Histone Chaperone AtHIRA is Involved in Somatic Homologous Recombination and Salinity Response in Arabidopsis

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  • 1College of Life Sciences, Fudan University, Shanghai 200438, China
    2Department of Biochemistry, Fudan University, Shanghai 200438, China

Received date: 2017-03-22

  Accepted date: 2017-08-30

  Online published: 2017-08-30

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Abstract

Histone regulator (HIRA) is a specific chaperone for histone H3.3 and plays an important role in the incorporation of histone H3.3 into chromatin. HIRA is indispensable in mammalian embryo development and DNA damage repair process, but we have few studies of the function of an HIRA homolog in plants. Here, we studied the function of Arabidopsis thaliana AtHIRA in somatic homologous recombination (HR) and meiotic homologous recombination. We used the somatic HR system and the meiotic homologous recombination system in wild type and the hira-1 mutant, a loss- of-function mutant of AtHIRA. Both intramolecular and intermolecular HR frequency was lower in hira-1 than the wild type under normal growth conditions and under bleomycin or UV-C treatment, with no significant difference in the frequency of meiotic recombination of microsporocytes between the wild type and hira-1 mutant under normal growth conditions. As well, under normal growth conditions or bleomycin treatment, loss-of-function of AtHIRA in Arabidopsis did not affect the DNA damage level. On qRT-PCR, the expression of RAD51 and RAD54, two DNA repair-related genes, was higher in hira-1 than the wild type. In addition, hira-1 had a salt-sensitive phenotype as compared with the wild type under salt stress. AtHIRA may play a role in somatic HR and the salinity response in Arabidopsis.

Key words： HIRA; homologous recombination; DNA damage repair; meiotic; salt stress

Cite this article

Cheng Chen, Aiwu Dong, Wei Su . Histone Chaperone AtHIRA is Involved in Somatic Homologous Recombination and Salinity Response in Arabidopsis[J]. Chinese Bulletin of Botany, 2018 , 53(1) : 42 -50 . DOI: 10.11983/CBB17058

References

[1] 夏志强, 何奕昆, 鲍时来, 种康 (2007). 植物开花的组蛋白甲基化调控分子机理. 植物学通报 24, 275-283.
[2] Amin AD, Vishnoi N, Prochasson P (2012). A global requirement for the HIR complex in the assembly of chromatin.Biochim Biophys Acta 1819, 264-276.
[3] Andersen SL, Sekelsky J (2010). Meiotic versus mitotic recombination: two different routes for double-strand b- reak repair: the different functions of meiotic versus mi- totic DSB repair are reflected in different pathway usage and different outcomes. Bioessays 32, 1058-1066.
[4] Francis KE, Lam SY, Harrison BD, Bey AL, Berchowitz LE, Copenhaver GP (2007). Pollen tetrad-based visual assay for meiotic recombination in Arabidopsis. Proc Natl Acad Sci USA 104, 3913-3918.
[5] Fritsch O, Benvenuto G, Bowler C, Molinier J, Hohn B (2004). The INO80 protein controls homologous recombination in Arabidopsis thaliana. Mol Cell 16, 479-485.
[6] Gao J, Zhu Y, Zhou WB, Molinier J, Dong AW, Shen WH (2012). NAP1 family histone chaperones are required for somatic homologous recombination in Arabidopsis.Plant Cell 24, 1437-1447.
[7] Gherbi H, Gallego ME, Jalut N, Lucht JM, Hohn B, White CI (2001). Homologous recombination in planta is stimulated in the absence of Rad50.EMBO Rep 2, 287-291.
[8] Giraut L, Falque M, Drouaud J, Pereira L, Martin OC, Mézard C (2011). Genome-wide crossover distribution in Arabidopsis thaliana meiosis reveals sex-specific patterns along chromosomes. PLoS Genet 7, e1002354.
[9] Heyer WD, Ehmsen KT, Liu J (2010). Regulation of homologous recombination in eukaryotes.Annu Rev Genet 44, 113-139.
[10] Ingouff M, Rademacher S, Holec S, Šoljić L, Xin N, Readshaw A, Foo SH, Lahouze B, Sprunck S, Berger F (2010). Zygotic resetting of the HISTONE 3 variant re- pertoire participates in epigenetic reprogramming in Arabi- dopsis.Curr Biol 20, 2137-2143.
[11] Krejci L, Altmannova V, Spirek M, Zhao XL (2012). Homologous recombination and its regulation.Nucleic Acids Res 40, 5795-5818.
[12] Loppin B, Bonnefoy E, Anselme C, Laurencon A, Karr TL, Couble P (2005). The histone H3.3 chaperone HIRA is essential for chromatin assembly in the male pronucleus.Nature 437, 1386-1390.
[13] Melamed-Bessudo C, Levy AA (2012). Deficiency in DNA methylation increases meiotic crossover rates in euchro- matic but not in heterochromatic regions in Arabidopsis.Proc Natl Acad Sci USA 109, E981-E988.
[14] Molinier J, Ries G, Bonhoeffer S, Hohn B (2004). Interchromatid and interhomolog recombination in Arabidopsis thaliana. Plant Cell 16, 342-352.
[15] Nie X, Wang HF, Li J, Holec S, Berger F (2014). The HIRA complex that deposits the histone H3.3 is conserved in Arabidopsis and facilitates transcriptional dynamics.Biol Open 3, 794-802.
[16] Okada T, Endo M, Singh MB, Bhalla PL (2005). Analysis of the histoneH3 gene family in Arabidopsis and identification of the male-gamete-specific variant AtMGH3. Plant J 44, 557-568.
[17] Otero S, Desvoyes B, Gutierrez C (2014). Histone H3 dynamics in plant cell cycle and development.Cytogenet Genome Res 143, 114-124.
[18] Roberts C, Sutherland HF, Farmer H, Kimber W, Halford S, Carey A, Brickman JM, Wynshaw-Boris A, Scambler PJ (2002). Targeted mutagenesis of theHira gene results in gastrulation defects and patterning abnormalities of mesoendodermal derivatives prior to early embryo- nic lethality. Mol Cell Biol 22, 2318-2328.
[19] Schuermann D, Fritsch O, Lucht JM, Hohn B (2009). Replication stress leads to genome instabilities in Arabidopsis DNA polymerase δ mutants.Plant Cell 21, 2700-2714.
[20] Schuermann D, Molinier J, Fritsch O, Hohn B (2005). The dual nature of homologous recombination in plants.Tren- ds Genet 21, 172-181.
[21] Sherwood PW, Osley MA (1991). Histone regulatory (hir) mutations suppress δ insertion alleles in Saccharomyces cerevisiae. Genetics 128, 729-738.
[22] Szenker E, Lacoste N, Almouzni G (2012). A developmental requirement for HIRA-dependent H3.3 deposition revealed at gastrulation in Xenopus. Cell Rep 1, 730-740.
[23] Tang Y, Poustovoitov MV, Zhao KH, Garfinkel M, Canu- tescu A, Dunbrack R, Adams PD, Marmorstein R (2006). Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly.Nat Struct Mol Biol 13, 921-929.
[24] Wijeratne AJ, Ma H (2007). Genetic analyses of meiotic recombination in Arabidopsis. J Integr Plant Biol 49, 1199-1207.
[25] Xu Y, Price BD (2011). Chromatin dynamics and the repair of DNA double strand breaks.Cell Cycle 10, 261-267.
[26] Zhu Y, Dong AW, Meyer D, Pichon O, Renou JP, Cao KW, Shen WH (2006). Arabidopsis NRP1 and NRP2 encode histone chaperones and are required for maintaining postembryonic root growth. Plant Cell 18, 2879-2892.
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