Characterisation and Gene Mapping of a Brittle Culm Mutant bc-s1 in Rice
Received date: 2015-03-06
Revised date: 2015-06-23
Online published: 2016-03-31
The mechanical strength of rice stem is an important agronomic trait that affects crop lodging. In this study, a brittle culm mutant, bc-s1, was identified from the rice mutant library. The mutant showed inherited traits of brittleness from bolting to mature stage. Meanwhile, the leaf, leaf sheath, culm, node and seed shell of the mutant showed brittleness during the mature stage. Compared with breaking and elongation force in the wild type, that in bc-s1 culms decreased 31.1% and 67.2%, respectively. Besides showing irregular shape of sclerenchyma cells, in bc-s1, cellulose and lignin content in culm decreased 24.97% and increased 38.82%, respectively. The BC-S1 gene was mapped on chromosome 9 between InDel markers Ha6 and Ha9 within 101 kb physical distance. According to sequencing results for bc-s1, a candidate open reading frame (Os09g25490/OsCesA9), which encodes a cellulose synthase catalytic subunit (CesA), showed a point mutation at the 28th base of first extron, changing GGG to AGG and causing the substitution at the 10th amino acid residue from glycine to arginine. Therefore, bc-s1 is a new allele of OsCesA9 and provides a new material for uncovering the biological function of OsCesA9 in rice cell-wall biosynthesis.
Key words： bc-s1; brittle culm; secondary wall; cellulose synthase; rice
Zhenming Jin, Baozhe Ping, Haojun Shen, Huaiqing Du, Ruiqian Li, Lu Zhu, Dabing Zhang, Zheng Yuan . Characterisation and Gene Mapping of a Brittle Culm Mutant bc-s1 in Rice[J]. Chinese Bulletin of Botany, 2016 , 51(2) : 167 -174 . DOI: 10.11983/CBB15048
[1]Brown Jr RM, Saxena IM.Cellulose biosynthesis: a model for understanding the assembly of biopolymers[J].Plant Physiology and Biochemistry, 2000, 38(1):57-67
[2]Doblin MS, Kurek I, Jacob-Wilk D, Delmer DP.Cellulose biosynthesis in plants: from genes to rosettes[J].Plant and Cell Physiology, 2002, 43(12):1407-1420
[3]Freemont PS.Ubiquitination: RING for destruction?[J].Current Biology, 2000, 10(2):R82-R87
[4]Harholt J, Jensen JK, S?rensen SO, Orfila C, Pauly M, Scheller HV.ARABINAN DEFICIENT 1 is a putative arabinosyltransferase involved in biosynthesis of pectic arabinan in Arabidopsis[J].Plant physiology, 2006, 140(1):49-58
[5]Hoebler C, Barry L, Delort-Laval J.Rapid hydrolysis of plant cell wall polysaccharides by gas-liquid chromatography[J]., 1989, 37(2):360-367
[6]Islam MS, Peng S, Visperas RM, Ereful N, Bhuiya M, Julfiquar A.Lodging-related morphological traits of hybrid rice in a tropical irrigated ecosystem[J].Field crops research, 2007, 101(2):240-248
[7]Joazeiro CA, Weissman AM.RING finger proteins: mediators of ubiquitin ligase activity[J].Cell, 2000, 102(5):549-552
[8]Kirk TK, Obst JR.Lignin determination[J].Methods in enzymology-Biomass, part b, lignin, pectin, and chitin,, 1988, 161:87-101
[9]Kotake T, Aohara T, Hirano K, Sato A, Kaneko Y, Tsumuraya Y, Takatsuji H, Kawasaki S.Rice Brittle culm 6 encodes a dominant-negative form of CesA protein that perturbs cellulose synthesis in secondary cell walls[J].Journal of experimental botany, 2011, 62(6):2053-2062
[10]Kurek I, Kawagoe Y, Jacob-Wilk D, Doblin M, Delmer D.Dimerization of cotton fiber cellulose synthase catalytic subunits occurs via oxidation of the zinc-binding domains[J].Proceedings of the National Academy of Sciences, 2002, 99(17):11109-11114
[11]Li Y, Qian Q, Zhou Y, Yan M, Sun L, Zhang M, Fu Z, Wang Y, Han B, Pang X.BRITTLE CULM1,which encodes a COBRA-like protein,affects the mechanical properties of rice plants[J].The Plant Cell, 2003, 15(9):2020-2031
[12]Murray M, Thompson WF.Rapid isolation of high molecular weight plant DNA[J].Nucleic acids research, 1980, 8(19):4321-4326
[13]Scheible W-R, Eshed R, Richmond T, Delmer D, Somerville C.Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in Arabidopsis Ixr1 mutants[J].Proceedings of the National Academy of Sciences, 2001, 98(18):10079-10084
[14]Song X-Q, Liu L-F, Jiang Y-J, Zhang B-C, Gao Y-P, Liu X-L, Lin Q-S, Ling H-Q, Zhou Y-H.Disruption of Secondary Wall Cellulose Biosynthesis Alters Cadmium Translocation and Tolerance in Rice Plants[J].Molecular plant, 2013, 6(3):768-780
[15]Tanaka K, Murata K, Yamazaki M, Onosato K, Miyao A, Hirochika H.Three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall[J].Plant Physiology, 2003, 133(1):73-83
[16]Timmers J, Vernhettes S, Desprez T, Vincken J-P, Visser RG, Trindade LM.Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall[J].FEBS letters, 2009, 583(6):978-982
[17]Updegraff DM.Semimicro determination of cellulose in biological materials[J].Analytical biochemistry, 1969, 32(3):420-424
[18]Wang D, Yuan S, Yin L, Zhao J, Guo B, Lan J, Li X.A missense mutation in the transmembrane domain of CESA9 affects cell wall biosynthesis and plant growth in rice[J].Plant Science, 2012, 196:117-124
[19]Zhang B, Zhou Y.Rice Brittleness Mutants: A Way to Open the ‘Black Box’of Monocot Cell Wall Biosynthesis[J].Journal of integrative plant biology, 2011, 53(2):136-142
[20]Zhong R, Ye ZH.Regulation of cell wall biosynthesis[J].Current opinion in plant biology, 2007, 10(6):564-572
[21]陈亮, 储黄伟, 袁政, 潘爱虎, 梁婉琪, 黄海, 沈明山, 张大兵.射线诱变水稻突变体的分离和遗传学初步分析[J].厦门大学学报: 自然科学版, 2006, 45(B05):82-85
[22]江云珠, 沈希宏, 曹立勇.水稻茎秆性状的研究进展[J].中国稻米, 2012, 18(2):1-7
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