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- 1Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, China;
2Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Key Laboratory for Genetics and Breeding of Banana, Haikou 570102, China
Received date: 2013-09-26
Revised date: 2014-03-21
Online published: 2014-07-02
Abstract
ASR (abscisic acid [ABA], stress, ripening-induced) proteins are a class of transcriptional factors involved in drought stress tolerance in many plant species. However, the regulation mechanism of ASR in banana under drought stress tolerance is not known. Previously, we screened a new ASR gene from the cDNA library of banana (Musa acuminata cv. ‘Brazilian’), designated MaASR1 (accession no. AY628102). MaASR1 expression was higher in leaves than roots under drought stress. MaASR1 was transferred into Arabidopsis thaliana and two independent transgenic lines (L14, L38) were obtained. The leaves of MaASR1 transgenic lines were smaller and thicker than those of the wild type. Survival rate and proline content were higher in L14 and L38 than in the wild type after drought treatment. Under drought stress and ABA treatment, We found that MaASR1 could enhance the sensitivity of the transgenic plants to ABA signal but did not improve ABA-dependent drought stress tolerance.
Cite this article
Hongxia Miao, Yuan Wang, Biyu Xu, Juhua Liu, Caihong Jia, Jianbin Zhang, Zhuo Wang, Peiguang Sun, Zhiqiang Jin . The Role of Banana MaASR1 in Drought Stress Tolerance[J]. Chinese Bulletin of Botany, 2014 , 49(5) : 548 -559 . DOI: 10.3724/SP.J.1259.2014.00548
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张治安, 陈展宇(2008). 植物生理学实验技术. 长春: 吉林大学出版社. pp. 192-194.
陈建辉, 李荣华, 郭培国, 夏岩石, 田长恩, 缪绅裕(2011). 干旱胁迫对不同耐旱性大麦品种叶片超微结构的影响. 植物学报46, 28-36.
Sambrook J, Russell D (黄培堂等译) (2002). 分子克隆实验指南(第3版). 北京: 科学出版社. pp. 492-509.
Amitai-Zeigerson H, Scolnik PA, Bar-Zvi D (1995). Tomato Asr1 mRNA and protein are transiently expressed following salt stress, osmotic stress and treatment with abscisic acid. Plant Sci 110, 205-213.
Clough SJ, Bent AF (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16, 735-743.
Cortés AJ, Chavarro MC, Madri?án S, This D, Blair MW (2012). Molecular ecology and selection in the drought-related Asr gene polymorphisms in wild and cultivated common bean (Phaseolus vulgaris L.). BMC Genet 13, 58-71.
Dóczi R, Csanaki C, Bánfalvi Z (2002). Expression and promoter activity of the desiccation-specific Solanum tuberosum gene, StDS2. Plant Cell Environ 25, 1197-1203.
Fischer I, Camus-kulandaivelu L, Allal F, Stephan W (2011). Adaptation to drought in two wild tomato species: the evolution of the Asr gene family. New Phytol 190, 1032-1044.
Hu W, Huang C, Deng X, Zhou S, Chen L, Li Y, Wang C, Ma Z, Yuan Q, Wang Y, Cai R, Liang X, Yang G, He G (2013). TaASR1, a transcription factor gene in wheat, confers drought stress tolerance in transgenic tobacco. Plant Cell Environ 36: 1449-1464.
Joo J, Lee Y H, Choi D H, Cheong J, Kim Y, Song S (2013).Rice ASR1 has function in Abiotic stress tolerance during early growth stages of rice. J Korean Soc Appl Biol Chem 56, 349-352.
Sakuma Y, Maruyama K, Yamaguchi-Shinozakia K (2006). Functional Analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. Plant Cell 18, 1292-1309.
Silhavy D, Hutvagner G, Barta E, Bánfalvi Z (1995). Isolation and characterization of a water-stress-inducible cDNA clone from Solanum chacoense. Plant Mol Biol 27, 587-595.
Thomashow MF (1999). Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms. Annu Rev Plant Physiol Plant Mol Biol 50, 571-599.
Padmanabhan V, Dias DMAL, Newton RJ (1997). Expression analysis of a gene family in loblolly pine (Pinus taeda L.) induced by water deficit stress. Plant Mol Biol 35, 801-807.
Vaidyanathan R, Kuruvilla S, Thomas G (1999).Characterization and expression pattern of an abscisic acid and osmotic stress responsive gene from rice. Plant Sci 140, 25-36.
Wang W, Vinocur B, Altman A (2003). Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218, 1-14.
Xiong L, Ishitani M, Lee H, Zhu JK (2001).The Arabidopsis LOS5/ABA3 locus encodes a molybdenum cofactor sulfurase and modulates cold stress-and osmotic stress-responsive gene expression. Plant Cell 13, 2063-2083.
Xiong L, Zhu JK (2003). Regulation of abscisic acid biosynthesis. Plant Physiol 133, 29-36.
Xu BY, Su W, Liu JH, Wang JB, Jin ZQ (2007). Differentially expressed cDNAs at the early stage of banana ripening identified by suppression subtractive hybridization and cDNA microarray. Planta 226, 529~539.
Yamaguchi-Shinozakiaib K, Shinozaki K (1994). A nove1 cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low temperature, or high-salt stress. Plant Cell 6, 251-264
Zhang ZB, Xu P, Shao HB, Liu MJ, Fu ZY, Chu LY (2011). Advances and prospects: biotechnologically improving crop water use efficiency. Crit Rev Biotechnol 31, 281-293.
Zhu J, Huang X, Liu T, Gao S, Chen J (2012). Cloning and function analysis of a drought-inducible gene associated with resistance to Curvularia leaf spot in maize. Mol Biol Rep 39, 7919-7926.