Chinese Bulletin of Botany >

2014 , Vol. 49 >Issue 6: 738 - 750

DOI: https://doi.org/10.3724/SP.J.1259.2014.00738

Recent Advances in Metabolic Products of Flavonoids in Nelumbo

Expand
  • 1Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;

    2University of Chinese Academy of Sciences, Beijing 100049, China;

    3College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2013-10-30

  Revised date: 2013-12-10

  Online published: 2014-11-21

Fold

Abstract

Nelumbo plants contain edible and pharmaceutical products; nelumbinis folium, rhizomatis nodus, semen, plumula, receptaculum, and stamen are all used in medicine. The efficacy of the medicine varies greatly between different parts. In total, 61 flavonoids, including 8 anthocyanins, 2 flavonols, 29 flavonol glycosides, 1 flavone, 16 flavone glycosides (13 C-glycosyl flavones) and 5 flavanols, have been identified in various lotus tissues. These flavonoid compounds are widely used in the health-food and medicine industries. This paper summarizes the recent progress in the detection and purification, composition, content and pharmacological studies of flavonoids in different Nelumbo tissues, comprising lotus leaves, petals, stamens, pistils, tori, leaf veins, leaf stalk, flower stalk, seed pods, seed coats, kernels, plumules and rhizome. Petals and stamens possess plentiful quercetin and kaempferol glycosides; quercetin glycosides are dominant components in other tissues except for plumules. Abundant C-glycosyl flavones were identified in plumules. In addition, the total content of flavonoids was significantly high in leaves. HPLC-DAD and HPLC-ESI-MSn are becoming the main detection methods because of their high sensitivity and accuracy. The most effective method for separating and purifying the single compound is high-speed counter-current chromatography (HSCCC). Because of the wide range of biological activities of Nelumbo tissues, including antioxidant, antibiotic, anti-HIV, anti-atherothrombotic, anti-inflammatory and anti-obesity properties, promoting the comprehensive study and development of the nutritional and pharmacodynamic material in each tissue is important.

Cite this article

Shanshan Li, Qian Wu, Ruyu Yuan, Shuai Shao, Huijin Zhang, Liangsheng Wang . Recent Advances in Metabolic Products of Flavonoids in Nelumbo[J]. Chinese Bulletin of Botany, 2014 , 49(6) : 738 -750 . DOI: 10.3724/SP.J.1259.2014.00738

References

陈健芬, 何卫华, 钱伏刚 (2003). 荷叶提取物口腔抑菌有效成分的定性定量分析. 日用化学工业 33, 49-51.
陈莎 (2013). 莲类黄酮和生物碱分析方法的建立、品种资源组分和含量的构成特点. 博士论文. 武汉: 中国科学院武汉植物园. pp. 56-73.
陈卫航, 谭美亭, 张婕 (2012). 响应曲面法优化莲房原花青素提取工艺研究. 郑州大学学报 (工学版) 33, 31-35.
陈轩, 周坚 (2011). 莲子皮化学成分的初步分析. 农业机械 20, 139-141.
单世斌, 沈元帅, 杨金, 丁骁, 陈启建 (2012). 莲子心中黄酮类物质的提取和鉴定. 海峡药学 24, 42-45.
段玉清, 张海晖, 唐瑛, 吴佳, 谢笔钧 (2005). 莲房原花青素对60Co-γ射线致亚急性辐射损伤防护的研究. 营养学报 27, 491-493.
郭明, 杨群超, 朱灵芝, 吕圭源 (2009). 荷花不同部位总黄酮分布及荷叶黄酮提取工艺研究. 中国现代应用药学 26, 211-215.
林宣贤 (2007). 荷花黄酮类的提取及其生物活性的研究. 中国食品添加剂 3, 65-68.
林志钦 (2012). 莲子心总黄酮的提取纯化及其功能性研究. 硕士论文. 福州: 福建农林大学. pp. 43-50.
凌智群 (2001). 莲房原花青素及其生物, 药理活性研究. 博士论文. 武汉: 华中农业大学. pp. 33-66.
苏本华, 苏春英, 孙静 (2011). 分光光度法测定莲须中总黄酮的含量. 中医药信息 28, 81-82.
王俊杰, 舒洋, 龙婷, 吴海燕, 陈福春, 方会龙, 蒋显勇 (2011). 荷叶黄酮治疗小鼠非酒精性脂肪肝的研究. 中药药理与临床 27, 61-64.
肖贵平 (2011a). 建莲莲心黄酮类物质水浸提条件优化. 亚热带农业研究 7, 192-196.
肖贵平 (2011b). 响应面法优化莲心黄酮类物质微波提取工艺的研究. 热带作物学报 32, 2163-2168.
肖俊松, 谢笔钧, 曹雁平, 吴华, 王成涛 (2012). 莲房原花色素的纯化分级和结构鉴定. 食品科学 33, 172-177.
许金蓉, 王清章 (2011). 莲藕中黄酮含量与分布研究. 湖北农业科学 50, 386-388.
徐静, 郭长江, 韦京豫, 杨继军, 程霜, 吴健全 (2005). 蔬菜中类黄酮物质的高效液相色谱测定法. 营养学报 27, 267-279.
徐双双, 刘伟, 郭英慧, 王萍, 孙瑜, 刘建华, 王晓 (2012). 高效液相色谱-四级杆飞行时间串联质谱分析3种荷花的花色苷. 山东科学 25, 66-70.
徐双双, 孙瑜, 井凤, 段文娟, 杜金华, 王晓 (2011). 硅胶柱色谱结合高速逆流色谱法分离纯化荷花中黄酮类化合物. 色谱 29, 1244-1248.
薛冬娜 (2008). 荷叶总黄酮提取纯化工艺及减肥降脂作用的研究. 硕士论文. 重庆: 西南大学. pp. 23-39.
杨冀艳, 胡磊, 许杨 (2009). Plackett-Burman设计和响应面法优化荷叶总黄酮的提取工艺. 食品科学 30, 29-33.
杨瑞珍 (2009). 荷花花色素分析及花色形成的化学机制. 学位论文. 北京: 中国科学院植物研究所. pp. 15-35.
禹华娟, 孙智达, 谢笔钧 (2010). 酶辅助提取莲房原花青素工艺及其抗氧化活性研究. 天然产物研究与开发 22, 154-158.
张胜帮, 叶静, 陈聪 (2012). 复合酶酶法提取荷叶黄酮类化合物工艺优化. 食品科学 33, 149-153.
张行言. (2005). 中国荷花新品种图志Ⅰ. 北京: 中国林业出版社. pp. 20-28.
赵昕岚, 邓放明 (2013). 荷叶中总黄酮和荷叶碱含量变化规律. 食品与机械 29, 37-40.
中华人民共和国卫生部药典委员会. (1985). 中华人民共和国药典(2010版). 北京: 化学工业出版社. pp. 256-257, 358.
朱满兰, 王亮生, 张会金, 徐彦军, 郑绪辰, 王丽金 (2012). 耐寒睡莲花瓣中花青素苷组成及其与花色的关系. 植物学报 47, 437-453.
Abad-Garcia B, Berrueta LA, Garmon-Lobato S, Gallo B. Vicente F (2009). A general analytical strategy for the characterization of phenolic compounds in fruit juices by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry. J Chromatogr A 1216, 5398-5415.
Agnihotri VK, ElSohly HN, Khan SI, Jacob MR, Joshi VC, Smillie T, Khan IA, Walker LA (2008). Constituents of Nelumbo nucifera leaves and their antimalarial and antifungal activity. Phytochem. Lett. 1, 89-93.
Bomser J, Madhavi D, Singletary K, Smith M (1996). In vitro anticancer activity of fruit extracts from Vaccinium species. Planta medica 62, 212-216.
Bravo L (1998). Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr. Rev. 56, 317-333.
Chen S, Fang LC, Xi HF, Guan L, Fang JB, Liu YL, Wu BH, Li SH (2012a). Simultaneous qualitative assessment and quantitative analysis of flavonoids in various tissues of lotus (Nelumbo nucifera) using high performance liquid chromatography coupled with triple quad mass spectrometry. Anal Chim Acta 724, 127-135.
Chen S, Wu BH, Fang JB, Liu YL, Zhang HH, Fang LC, Guan L, Li SH (2012b). Analysis of flavonoids from lotus (Nelumbo nucifera) leaves using high performance liquid chromatography/photodiode array detector tandem electrospray ionization mass spectrometry and an extraction method optimized by orthogonal design. J Chromatogr A 1227, 145-153.
Chen S, Xiang Y, Deng J, Liu YL, Li SH (2013). Simultaneous analysis of anthocyanin and non-anthocyanin flavonoid in various tissues of different lotus (Nelumbo) cultivars by HPLC-DAD-ESI-MSn. PloS one 8, e62291.
Deng J, Chen S, Yin XJ, Wang K, Liu YL, Li SH, Yang PF (2013). Systematic qualitative and quantitative assessment of anthocyanins, flavones and flavonols in the petals of 108 lotus (Nelumbo nucifera) cultivars. Food Chem 139, 307-312.
Deng SG, Deng ZY, Fan YW, Li J, Liu R, Xiong DM (2009a). Application of high-speed counter-current chromatography coupled with high performance liquid chromatography for the separation and purification of Quercetin-3-O-sambubioside from the leaves of Nelumbo nucifera. Front. Chem. Eng. China 3, 375-382.
Deng SG, Deng ZY, Fan YW, Peng Y, Li J, Xiong DM, Liu R (2009b). Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chromatography. J Chromatogr B Analyt Technol Biomed Life Sci 877, 2487-2492.
Goo HR, Choi JS, Na DH (2009). Simultaneous determination of quercetin and its glycosides from the leaves of Nelumbo nucifera by reversed-phase high-performance liquid chromatography. Arch Pharm Res 32, 201-206.
Guo XF, Wang DJ, Duan WJ, Du JH, Wang X (2010a). Preparative isolation and purification of four flavonoids from the petals of Nelumbo nucifera by high-speed counter-current chromatography. Phytochem Anal 21, 268-272.
Guo XF, Wang DJ, Duan WJ, Du JH, Wang X (2010b). Preparative isolation and purification of four flavonoids from the petals of Nelumbo nucifera by high‐speed counter‐current chromatography. Phytochem. Anal 21, 268-272.
Hu M, Skibsted LH (2002). Antioxidative capacity of rhizome extract and rhizome knot extract of edible lotus (Nelumbo nuficera). Food Chem, 76, 327-333.
Huang B, Ban XQ, He JS, Tong J, Tian J, Wang YW (2010). Hepatoprotective and antioxidant activity of ethanolic extracts of edible lotus (Nelumbo nucifera Gaertn.) leaves. Food Chem 120, 873-878.
Jung HA, Jung YJ, Yoon NY, Jeong DM, Bae HJ, Kim DW, Na DH, Choi JS (2008). Inhibitory effects of Nelumbo nucifera leaves on rat lens aldose reductase, advanced glycation endproducts formation, and oxidative stress. Food Chem Toxicol 46, 3818-3826.
Jung HA, Kim JE, Chung HY, Choi JS (2003). Antioxidant principles of Nelumbo nucifera stamens. Arch Pharm Res 26, 279-285.
Jurani? Z, ?i?ak ? (2005). Biological activities of berries: from antioxidant capacity to anti-cancer effects. Biofactors 23, 207-211.
Kashiwada Y, Aoshima A, Ikeshiro Y, Chen Y P, Furukawa H, Itoigawa M, Fujioka T, Mihashi K, Cosentino LM, Morris-Natschke SL, Lee KH (2005). Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure-activity correlations with related alkaloids. Bioorg Med Chem 13, 443-448.
Katori M, Watanabe K, Nomura K, Yoneda K (2002). Cultivar differences in anthocyanin and carotenoid pigments in the petals of the flowering lotus (Nelumbo spp.). J. Jpn. Soc. Hort. Sci 71, 812-817.
Kim MJ, Shin HS (2012). Antioxidative effect of lotus seed and seedpod extracts. Food Sci. Biotechnol 21, 1761-1766.
Kredy HM, Huang DH, Xie BJ, He H, Yang EN, Tian BQ, Xiao D (2010). Flavonols of lotus (Nelumbo nucifera, Gaertn.) seed epicarp and their antioxidant potential. Eur. Food Res. Technol 231, 387-394.
Li C, Du H, Wang L, Shu Q, Zheng Y, Xu Y, Zhang J, Zhang J, Yang R, Ge Y (2009). Flavonoid composition and antioxidant activity of tree peony (Paeonia section moutan) yellow flowers. J Agric Food Chem 57, 8496-8503.
Li, SS, Wu, J, Chen, LG, Du, H, Xu, YJ, Wang, LJ, Zhang, HJ, Zheng, XC, Wang, LS (2013). Biogenesis of C-glycosyl flavones and profiling of flavonoid glycosides in lotus (Nelumbo nucifera). Phytochem (accepted).
Lim SS, Jung YJ, Hyun SK, Lee YS, Choi JS (2006). Rat lens aldose reductase inhibitory constituents of Nelumbo nucifera stamens. Phytother Res 20, 825-830.
Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L (2004). Polyphenols: food sources and bioavailability. Am. J. Clin. Nutr 79, 727-747.
Ohkoshi E, Miyazaki H, Shindo K, Watanabe H, Yoshida A, Yajima H (2007). Constituents from the leaves of Nelumbo nucifera stimulate lipolysis in the white adipose tissue of mice. Planta medica, 73, 1255-1259.
Ono Y, Hattori E, Fukaya Y, Imai S, Ohizumi Y (2006). Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats. J Ethnopharmacol 106, 238-244.
Qi S, Zhou D (2013). Lotus seed epicarp extract as potential antioxidant and anti-obesity additive in Chinese Cantonese Sausage. Meat Sci 93, 257-262.
Rahman W, Ilyas M, Khan AW (1962). Flower pigments: Flavonoids from nelumbo nucifera Gaerten. Naturwissenschaften 49, 327-327.
Rai S, Wahile A, Mukherjee K, Saha BP, Mukherjee PK (2006). Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds. J Ethnopharmacol 104, 322-327.
Yang RZ, Wei XL, Gao FF, Wang LS, Zhang HJ, Xu YJ, Li CH, Ge YX, Zhang JJ, Zhang J (2009). Simultaneous analysis of anthocyanins and flavonols in petals of lotus (Nelumbo) cultivars by high-performance liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry. J Chromatogr A 1216, 106-112.
Zafra-Stone S, Yasmin T, Bagchi M, Chatterjee A, Vinson JA, Bagchi D (2007). Berry anthocyanins as novel antioxidants in human health and disease prevention. Mol. Nutr. Food Res 51, 675-683.
Zhang L, Shan Y, Tang KJ, Putheti R (2009). Ultrasound-assisted extraction flavonoids from Lotus (Nelumbo nuficera Gaertn) leaf and evaluation of its anti-fatigue activity. Int. J. Phys 4, 418-422.
Zhu ML, Zheng XC, Shu QY, Li H, Zhong PX, Zhang HJ, Xu YJ, Wang LJ, Wang LS (2012). Relationship between the composition of flavonoids and flower colors variation in tropical water lily (Nymphaea) cultivars. PloS one 7, e34335.
Options
Outlines

/

〈 〉

Copyright © Chinese Bulletin of Botany, All Rights Reserved.
Powered by Beijing Magtech Co. Ltd
674-3466/bottom_en.htm"-->