Cloning of Flowering-related Gene AcMADS1 and Characterization of Expression in Tissues of Pineapple (Ananas comosus)
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- 1Guangxi Subtropical Crops Research Institute, Nanning 530001, China;
2South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China;
3Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;
4College of Horticulture and Landscape Architecture, Hainan University, Haikou 570228, China;
5Hainan Academy of Agricultural Sciences, Haikou 571100, China
Received date: 2013-05-07
Revised date: 2013-11-03
Online published: 2014-11-21
Abstract
MADS-box gene is a key transcription factor that plays crucial roles in plant development, especially controlling the formation and development of floral organs. We cloned the flower-specific gene from floral organs of Ananas comosus, named AcMADS1 (GeneBank accession no. KC257408), was cloned by homology-based cloning and RACE. The open reading frame of AcMADS1 was 726 bp, encoding 241 amino acids with molecular weight 27.5 kDa and isoelectric point 9.26. Sequence alignment and phylogenetic tree analysis indicated that the deduced protein AcMADS1 contained a conservative MADS-box and semi-conservative K domain and belonged to the AGL6 clade of the MADS-box family. Bioinformatics analysis demonstrated that AcMADS1 was an alkaline and hydrophilic protein; the secondary structure comprised an α-helix, a random coil and an extended strand; the protein core structure agreed with the transcription factors and the function of the common DNA combining domain MADS-box in its protein 3D model and was located in the nucleus as a transcription factor. Tissue-specific analysis showed that AcMADS1 was expressed in floral organs (pistils, petals and sepals) and flesh of pineapple, with nearly no transcription detected in vegetative organs (roots, stems and leaves) and stamens. Furthermore, the expession of AcMADS1 was higher at the early phase of floral development, then reduced at the late phase. AcMADS1 may have important roles in floral induction and floral development of pineapple.
Cite this article
Yuanbao Cai, Xiangyan Yang, Guangming Sun, Qiang Huang, Yeqiang Liu, Shaopeng Li, Zhili Zhang . Cloning of Flowering-related Gene AcMADS1 and Characterization of Expression in Tissues of Pineapple (Ananas comosus)[J]. Chinese Bulletin of Botany, 2014 , 49(6) : 692 -703 . DOI: 10.3724/SP.J.1259.2014.00692
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[4]Angenent GC,Franken J,Busscher M,van Dijken A,van Went JL,Dons HJ,van Tunen AJ.A novel class of MADS box genes is involved in ovule development in petunia[J].Plant Cell,1995,7:1569-1582
[5]Becker A,Thei?en G.The major clades of MADS-box genes and their role in the development and evolution of flowering plants[J].Mol Phylogenet Evol,2003,29:464-489
[6]Boss PK,Sensi E,Hua C,Davies C,Thomas MR.Cloning and characterisation of grapevine (Vitis vinifera L[J].) MADS-box genes expressed during inflorescence and berry development. Plant Sci,2002,162:887-895
[7]Coen ES,Meyerowitz EM.The war of the whorls: genetic interactions controlling flower development[J].Nature,1991,353:31-37
[8]de Folter S,Immink RGH,Kieffer M,Parenicova L,Henz SR,Weigel D,Busscher M,Koolker M,Colombo L,Kater MM,Davies B,Angenent GC.Comprehensive interaction map of the Arabidopsis MADS-box transcription factors[J].Plant Cell,2005,17:1424-1433
[9]Ferrario S,Immink RG,Angenent GC.Conservation and diversity in flower land[J].Curr Opin Plant Biol,2004,7:84-91
[10]Gramzow L,Ritz MS,Thei?en G.On the origin of MADS-domain transcription factors[J].Trends Genet,2010,26:149-153
[11]Hsu HF,Huang CH,Chou LT,Yang CH.Ectopic expression of an orchid (Oncidium Gower Ramsey) AGL6-like gene promotes flowering by activating flowering time genes in Arabidopsis thaliana[J].Plant Cell Physiol,2003,44:783-794
[12]Immink RG,Gadella TWJ,Ferrario S,Busscher M,Angenent GC.Analysis of MADS box protein-protein interactions in living plant cells[J].P Natl Acad Sci USA,2002,99:2416-2421
[13]Irish VF (2003).The evolution of floral homeotic gene function. Bioessays,,25(7): 637–645
[14]Jack T.Molecular and genetic mechanisms of floral control[J].Plant Cell,2004,16:1-17
[15]Kaufmann K,Melzer R,Thei?en G.MIKC-type MADS-domain proteins: structural modularity,protein interactions and network evolution in land plants[J].Gene,2005,347:183-198
[16]Koo SC,Bracko O,Park MS,Schwab R,Chun HJ,Park KM,Seo JS,Grbic V,Balasubramanian S,Schmid M,Godard F,Yun DJ,Lee SY,Cho MJ,Weigel D,Kim MC.Control of lateral organ development and flowering time by the Arabidopsis thaliana MADS-box Gene AGAMOUS-LIKE6[J].Plant J,2010,62:807-816
[17]Li H,Liang W,Jia R,Yin C,Zong J,Kong H,Zhang D.The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice[J].Cell Res,2010,20:299-313
[18]Lin EP,Peng HZ,Jin QY,Deng MJ,Li T,Xiao XC,Hua XQ,Wang KH,Bian HW,Han N,Zhu MY.Identification and characterization of two bamboo (Phyllostachys praecox) AP1/SQUA-like MADS-box genes during floral transition[J].Planta,2009,231:109-120
[19]Liu C,Zhang J,Zhang N,Shan H,Su K,Zhang J,Meng Z,Kong H,Chen Z.Interactions among proteins of floral MADS-box genes in basal eudicots: implications for evolution of the regulatory network for flower development[J].Mol Biol Evol,2010,27:1598-1611
[20]Ma H,Yanofsky MF,Meyerowitz EM.AGL1-AGL6,an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes[J].Gene Dev,1991,5:484-495
[21]Melzer R,Wang YQ,Thei?en G.The naked and the dead: The ABC's of gymnosperm reproduction and the origin of the angiosperm flower[J].Semin Cell Dev Biol,2009,21:118-128
[22]Ohmori S,Kimizu M,Sugita M,Miyao A,Hirochika H,Uchida E,Nagato Y,Yoshida H.MOSAIC FLORAL ORGANS1,an AGL6-Like MADS box gene,regulates floral organ identity and meristem fate in rice[J].Plant Cell,2009,21:3008-3025
[23]Parenicová L,de Folter S,Kieffer M,Horner DS,Favalli C,Busscher J,Cook HE,Ingram RM,Kater MM,Davies B,Angenent GC,Colombo L.Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world[J].Plant Cell,2003,15:1538-1551
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[25]Reinheimer R,Kellogg EA.Evolution of AGL6-like MADS box genes in grasses (Poaceae): ovule expression is ancient and palea expression is new[J].Plant Cell,2009,21:2591-2605
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[32]Thompson BE,Bartling L,Whipple C,Hall DH,Sakai H,Schmidt R,Hake S.Bearded-ear encodes a MADS box transcription factor critical for maize floral development[J].Plant Cell,2009,21:2578-2590
[33]Tian B,Chen YY,Yan YX,Li DZ.Isolation and ectopic expression of a bamboo MADS-box gene[J].Chinese Sci Bull,2005,50:217-224
[34]Viaene T,Vekemans D,Becker A,Melzer S,Geuten K.Expression divergence of the AGL6 MADS domain transcription factor lineage after a core eudicot duplication suggests functional diversification.[J].BMC Plant Biol,2010,10:148-
[35]Weigel D,Meyerowitz EM.The ABCs of floral homeotic genes. [J].Cell,1994,78:203-209
[36]Yang Y,Fanning L,Jack T.The K domain mediates heterodimerization of the Arabidopsis floral organ identity proteins,APETALA3 and PISTILLATA[J].Plant J,2003,33:47-59