Poly ADP-ribose polymerase (PARP) is induced specifically by genotoxin. We cloned the 2 179 bp promoter sequence of Arabidopsis AtPARP1 gene into a pAKK687 vector to drive the expression of a β-glucuronidase (GUS) reporter gene for Arabidopsis transformation. GUS staining revealed the GUS reporter gene expressed only in the basal root of 3 to 5-day-old seedlings and in the anther at early flowering stage. An amount of 1.5 μg·mL–1 bleomycin combined
with 22 μg·mL–1 mitomycin could strongly induce the expression of the GUS reporter gene, especially in young seedlings and young siliques of Arabidopsis. An amount of 1 μg·mL–1 bleomycin was enough to induce the promoter and had no adverse effects on Arabidopsis seedling growth. The AtPARP1 promoter may be a new antibiotic-inducible promoter with application potential.
Weiwei Liu, Hailei Zhang, Caifeng Liu, Xiaochun Ge
. Construction and Activity Analysis of an Antibiotic-inducible Promoter[J]. Chinese Bulletin of Botany, 2011
, 46(5)
: 560
-568
.
DOI: 10.3724/SP.J.1259.2011.00560
吴雪峰, 赵开军, 陈毓荃 (2004). 植物启动子的诱导模序. 中国生物工程杂志 12, 14-21.
Ahel D, Ho?ej?í Z, Wiechens N, Polo SE, Garcia-Wilson E, Ahel I, Flynn H, Skehel M, West SC, Jackson SP, Owen-Hughes T, Boulton SJ (2009). Poly(ADP-ribose)–dependent regulation of DNA repair by the chromatin remodeling enzyme ALC1. Science 325, 1240-1243.
Althaus FR, Richter C (1987). ADP-ribosylation of proteins: enzymology and biological significance. Mol Biol Biochem Biophys 37 1–237.
Althaus FR (1992). Poly ADP-ribosylation: a histone shuttle mechanismin DNA excision repair. J Cell Sci 102, 663-670.
Ame JC, Spenlehauer C, de Murcia G (2004). The PARP superfamily. Bioessays 26, 882-893.
Barth E (2006). The world according to poly(ADP-ribose) polymerase (PARP) - update. Intens Care Med 32, 1470-1474.
Huber A, Bai P, de Murcia JM (2004). PARP-1, PARP-2 and ATM in the DNA damage response: functional synergy in mouse development. DNA Repair 3, 1103-1108.
Schreiber V, Dantzer F, Ame JC, de Murcia G (2006). Poly(ADP-ribose): novel functions for an old molecule. Nat Rev Mol Cell Bio 7, 517-528.
Petrucco S (2003). Sensing DNA damage by PARP-like fingers. Nucl Acids Res 31, 6689-6699.
Zuo J, Chua NH (2000). Chemical-inducible systems for regulated expression of plant genes. Curr Opin Biotech 11, 146-151.
Giandomenico C, Marianthi K (2009). Inducible gene expression systems and plant biotechnology. Biotech Adv 6, 733-743.
Mcelroy D, Brettell RIS (1994). Foreign gene-expression in transgenic cereals. Trends Biotech 12, 62-68.
Rossi FM, Blau HM (1998). Recent advances in inducible gene expression systems. Curr Opin Biotech 5, 451-456
Wang R, Zhou X, Wang X (2003). Chemically regulated expression systems and their applications in transgenic plants. Transgenic Res 12, 529–540.
Li R, Jia X, Mao X (2005). Ethanol-inducible gene expression system and its applications in plant functional genomics. Plant Sci 3, 463-469.
Tang W, Luo X, Samuels V (2004). Regulated gene expression with promoters responding to inducers. Plant Sci 4, 827-834.
Clough SJ, Bent AF (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743.
Xin ZG, Velten JP, Oliver MJ, Burke JJ (2003). High-throughput DNA extraction method suitable for PCR. Biotechniques 34, 1–6.
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