GATL12 is Essential for Chloroplast Biogenesis in Arabidopsis

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  • 1College of Basic Medicine and Biological Sciences, Soochow University, Suzhou 215123, China

    2Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

    3Center of Metabolism and Signal Transduction, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

    4 National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing 100081, China

Received date: 2011-03-09

  Revised date: 2011-04-13

  Online published: 2011-07-01

Abstract

Glutamine amidotransferase (GATase) catalyses the removal of the ammonia group from glutamine and then transfers this group to a substrate to form a new carbon-nitrogen group. Two classes of GATase domains have been identified: Class I (also known as trpG-type or triad) and Class II (also known as purF-type or Ntn). Arabidopsis genome contains 13 genes encoding Class-I-like proteins (GATLs) and their biological functions have not been reported. We isolated two T-DNA insertion mutants of GATL12 (gatl12-1 and gatl12-2) in which most of the ovules in the heterozygous mutants turned green on day 8 due to the chloroplast accumulation, but about 25% of the ovules were white. About 25% of heterozygous plant seeds cultured in 1/2MS medium developed into albino seedlings, which were homozygous mutants demonstrated by PCR. The transcripts of GATL12 could not be detected in the albino seedlings. Electron microscopy analysis showed that the chloroplasts could not develop in the mutants. Our results suggest that GATL12 plays an important role in chloroplast development in Arabidopsis.

Cite this article

Yanping Su, Xiaoduo Lu, Songdong Shen, Chunyi Zhang . GATL12 is Essential for Chloroplast Biogenesis in Arabidopsis[J]. Chinese Bulletin of Botany, 2011 , 46(4) : 379 -385 . DOI: 10.3724/SP.J.1259.2011.00379

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